Jiang Xiaoyan, Kurihara Toshihide, Ikeda Shin-Ichi, Kunimi Hiromitsu, Mori Kiwako, Torii Hidemasa, Tsubota Kazuo
Laboratory of Photobiology, Keio University School of Medicine; Department of Ophthalmology, Keio University School of Medicine.
Laboratory of Photobiology, Keio University School of Medicine; Department of Ophthalmology, Keio University School of Medicine;
J Vis Exp. 2019 Jan 22(143). doi: 10.3791/58822.
Murine model of myopia can be a powerful tool for myopia research because of the comparatively easy genetic manipulation. One way to induce myopia in animals is to put clear minus lenses in front of eyes for weeks (lens-induced myopia, LIM). However, extant protocols for inducement and evaluation vary from laboratory to laboratory. Here, we described a highly practical and reproducible method to induce LIM in mice using newly designed eyeglasses. The method fixes the lens stably in front of the mouse eye while allows the lens to be taken off for cleaning or topical drug administration. The phenotype is robust and efficient, and the variance is small. The method described here can be applied to mice right after weaning which extends the possible duration for experiments. We also gave technical advises for achieving reproducible results in refraction and axial length measurements. We hope the step-by-step protocol described here and the detailed article can help researchers perform myopia experiments with myopia more smoothly and make the data comparable across laboratories.
由于基因操作相对容易，近视小鼠模型可成为近视研究的有力工具。在动物中诱导近视的一种方法是在眼睛前放置透明负透镜数周（透镜诱导性近视，LIM）。然而，现有的诱导和评估方案在不同实验室之间存在差异。在此，我们描述了一种使用新设计的眼镜在小鼠中诱导LIM的高度实用且可重复的方法。该方法将透镜稳定地固定在小鼠眼前，同时允许取下透镜进行清洁或局部给药。表型强大且高效，方差小。这里描述的方法可在小鼠断奶后立即应用，这延长了可能的实验持续时间。我们还给出了在屈光和眼轴长度测量中获得可重复结果的技术建议。我们希望这里描述的分步方案和详细文章能帮助研究人员更顺利地进行近视实验，并使各实验室的数据具有可比性。
