Shepelin A P, Polosenko O V, Marchikhina I I, Sholokhova L P, Azhermacheva N I, Ershova M G, Poletaeva E D
FederalState Institution of Science State Research Center for Applied Microbiology & Biotechnology, Rospotrebnadzor, 142279, Obolensk, Serpukhov district, Moscow region, Russian Federation.
State budgetary institution of health care Infectious disease hospital, 150040 Yaroslavl, Russia.
Klin Lab Diagn. 2018;63(9):557-563. doi: 10.18821/0869-2084-2018-63-9-557-563.
Salmonella infections continue to be a serious problem in modern medicine. Being intestinal infections-associated pathogens, representatives of the genus Salmonella manifest themselves as pathogenic bacteria, especially in developing nosocomial infections. Given the polymorphism of clinical symptoms of salmonellosis, laboratory studies using bacteriological and serological methods are an important link in the diagnosis. In addition, the general prevention of salmonellosis includes measures to identify bacteria carriers, to ensure control over the incidence in farm animals and birds, food safety, etc. The list of nutrient media to isolate and identify Salmonella is lengthy and steadily extending, and the choice of specific media is largely relies on the nature of the material under study as well as on the idea of the potential availability of Salmonella bacteria in it, with research, diagnosis or epidemic situation being taken into account. The SRCAMB (Rospotrebnadzor) has designed two nutrient media allowing the enrichment and isolation Salmonella from various clinical samples. These are "Nutrient Medium for Enrichment of Salmonella, Dry (Magnesium medium) and "Nutrient Agar with Brilliant Green, Phenolic Red, Lactose and Sucrose, Dry (BPLS-FMH agar)." Growth and inhibitory properties of the new culture media produced by the SRCAMB and commercial domestic and foreign counterparts have been compared by using clinical material. Domestic nutrient media such as Magnesium medium and BPLS-FMH agar were proved to correspond to their commercial analogues when being used for enrichment, isolating and counting Salmonella bacteria in clinical specimens to have bacteriological control real data.
沙门氏菌感染在现代医学中仍然是一个严重问题。作为与肠道感染相关的病原体，沙门氏菌属的代表表现为病原菌，尤其是在医院感染的发生过程中。鉴于沙门氏菌病临床症状的多样性，采用细菌学和血清学方法的实验室研究是诊断中的重要环节。此外，沙门氏菌病的一般预防措施包括识别细菌携带者、确保对农场动物和禽类发病率的控制、食品安全等。用于分离和鉴定沙门氏菌的营养培养基种类繁多且不断增加，具体培养基的选择很大程度上取决于所研究材料的性质以及其中沙门氏菌的潜在可获得性，并需考虑研究、诊断或疫情情况。俄罗斯消费者权益保护和公益监督局（Rospotrebnadzor）设计了两种营养培养基，可用于从各种临床样本中富集和分离沙门氏菌。它们是“沙门氏菌富集干燥营养培养基（镁培养基）”和“含亮绿、酚红、乳糖和蔗糖的干燥营养琼脂（BPLS - FMH琼脂）”。通过使用临床材料，对俄罗斯消费者权益保护和公益监督局生产的新培养基以及国内外同类商业培养基的生长和抑制特性进行了比较。结果证明，国内的镁培养基和BPLS - FMH琼脂等营养培养基在用于临床标本中沙门氏菌的富集、分离和计数以获得细菌学对照真实数据时，与它们的商业类似物相当。
