Poisson D M
Laboratoire de Bactériologie du CHRO, Orléans, France.
Res Microbiol. 1992 Feb;143(2):211-6. doi: 10.1016/0923-2508(92)90010-l.
We describe a medium, novobiocin, brilliant green, glycerol, lactose (NBGL) agar, for the routine isolation of Salmonella strains from stool samples. The NBGL agar principle involved the use of the antisaprophytic effect of brilliant green and novobiocin. Glycerol and lactose were added in order to distinguish between Citrobacter and Salmonella. NBGL was used in parallel with salmonella-shigella (SS) and Hektoen (H) agar for culturing 2,853 stool samples, of which 184 were confirmed to be salmonellae. NBGL showed a high sensitivity: 94% in direct plating compared to 74% (p < 10(-3)) and 65% (p < 10(-5)) for H and SS, respectively, and 96% in enrichment broth plating vs. 83% (p < 10(-4)) and 86% (p < 10(-3)), respectively, for H and SS. In direct plating using NBGL, 95% of black-centred colonies were confirmed to be salmonellae (vs. 31% and 36%, H and SS). In enrichment plating using NBGL, this figure was 82% (vs. 26% and 28%). The results suggest that NBGL agar is advantageous for the isolation of non-Typhi H2S+ salmonellae.
我们描述了一种用于从粪便样本中常规分离沙门氏菌菌株的培养基——新生霉素、亮绿、甘油、乳糖（NBGL）琼脂。NBGL琼脂的原理是利用亮绿和新生霉素的抗腐生作用。添加甘油和乳糖是为了区分柠檬酸杆菌和沙门氏菌。将NBGL与沙门氏菌-志贺氏菌（SS）琼脂和赫克托恩（H）琼脂同时用于培养2853份粪便样本，其中184份被确认为沙门氏菌。NBGL显示出高灵敏度：直接平板接种时为94%，而H琼脂和SS琼脂分别为74%（p < 10⁻³）和65%（p < 10⁻⁵）；富集肉汤平板接种时为96%，而H琼脂和SS琼脂分别为83%（p < 10⁻⁴）和86%（p < 10⁻³）。在使用NBGL的直接平板接种中，95%的中心为黑色的菌落被确认为沙门氏菌（而H琼脂和SS琼脂分别为31%和36%）。在使用NBGL的富集平板接种中，这一数字为82%（而H琼脂和SS琼脂分别为26%和28%）。结果表明，NBGL琼脂有利于非伤寒H2S+沙门氏菌的分离。
