German Cancer Research Center (DKFZ), Heidelberg, Germany.
Heidelberg University, Heidelberg, Germany.
PLoS One. 2019 Feb 8;14(2):e0198149. doi: 10.1371/journal.pone.0198149. eCollection 2019.
Palmitoylation is an important posttranslational modification regulating diverse cellular functions. Consequently, aberrant palmitoylation can lead to diseases such as neuronal disorders or cancer. In humans there are roughly one hundred times more palmitoylated proteins than enzymes catalyzing palmitoylation (palmitoyltransferases). Therefore, it is an important challenge to establish the links between palmitoyltransferases and their targets. From publicly available data, we find that expression of human ZDHHC8 correlates significantly with cancer survival. To elucidate the organismal function of ZDHHC8, we study the Drosophila ortholog of hZDHHC8, CG34449/dZDHHC8. Knockdown of dZDHHC8 causes tissue overgrowth while dZDHHC8 mutants are larval lethal. We provide a list of 159 palmitoylated proteins in Drosophila and present data suggesting that scribble and Ras64B are targets of dZDHHC8.
棕榈酰化是一种重要的翻译后修饰，调节多种细胞功能。因此，异常的棕榈酰化可导致神经紊乱或癌症等疾病。在人类中，棕榈酰化蛋白的数量大约是催化棕榈酰化的酶（棕榈酰转移酶）的一百倍。因此，建立棕榈酰转移酶与其靶标的联系是一个重要的挑战。从公开数据中，我们发现人 ZDHHC8 的表达与癌症存活率显著相关。为了阐明 ZDHHC8 的机体功能，我们研究了 hZDHHC8 的果蝇同源物，CG34449/dZDHHC8。dZDHHC8 的敲低导致组织过度生长，而 dZDHHC8 突变体则是幼虫致死的。我们提供了一个在果蝇中 159 个棕榈酰化蛋白的列表，并提供了数据表明 scribble 和 Ras64B 是 dZDHHC8 的靶标。
