利用金纳米星胶体增强拉曼光谱法检测低浓度的二盐酸组胺
Detection of Histamine Dihydrochloride at Low Concentrations Using Raman Spectroscopy Enhanced by Gold Nanostars Colloids.
作者信息
Kolosovas-Machuca Eleazar Samuel, Cuadrado Alexander, Ojeda-Galván Hiram Joazet, Ortiz-Dosal Luis Carlos, Hernández-Arteaga Aida Catalina, Rodríguez-Aranda Maria Del Carmen, Navarro-Contreras Hugo Ricardo, Alda Javier, González Francisco Javier
机构信息
Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología, Universidad Autónoma de San Luis Potosí, 78210 San Luis Potosí, Mexico.
Applied Optics Complutense Group, Faculty of Optics and Optometry, University Complutense of Madrid, Av. Arcos de Jalon, 118, 28037 Madrid, Spain.
出版信息
Nanomaterials (Basel). 2019 Feb 6;9(2):211. doi: 10.3390/nano9020211.
In this paper, we report a fast and easy method to detect histamine dihydrochloride using gold nanostars in colloidal aqueous solution as a highly active SERS platform with potential applications in biomedicine and food science. This colloid was characterized with SEM and UV⁻Vis spectroscopy. Also, numerical calculations were performed to estimate the plasmonic resonance and electric field amplification of the gold nanoparticles to compare the difference between nanospheres and nanostars. Finally, aqueous solutions of histamine dihydrochloride were prepared in a wide range of concentrations and the colloid was added to carry out SERS. We found SERS amplified the Raman signal of histamine by an enhancement factor of 1 . 0 × 10 7 , demonstrating the capability of the method to detect low concentrations of this amine molecule.
在本文中，我们报道了一种快速简便的方法，该方法利用胶体水溶液中的金纳米星作为高活性表面增强拉曼散射（SERS）平台来检测二盐酸组胺，此平台在生物医学和食品科学领域具有潜在应用价值。通过扫描电子显微镜（SEM）和紫外可见光谱对该胶体进行了表征。此外，还进行了数值计算以估计金纳米颗粒的等离子体共振和电场放大，从而比较纳米球和纳米星之间的差异。最后，制备了一系列不同浓度的二盐酸组胺水溶液，并加入该胶体进行SERS检测。我们发现SERS将组胺的拉曼信号放大了1.0×10⁷倍，证明了该方法检测低浓度这种胺分子的能力。
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