Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/151, A-1060 Vienna, Austria; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, 71526 Assiut, Egypt.
Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, Vienna, Austria; Department of Plant Pathology, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt.
Talanta. 2015 Jun 1;138:183-189. doi: 10.1016/j.talanta.2015.02.022. Epub 2015 Feb 25.
A comparative evaluation of antimicrobial effect of synthesized silver nanoparticles (AgNPs) of different shapes using different methods was performed. Spherical, triangular and hexagonal AgNPs with an average size of 40 nm were chemically prepared and characterized by transmission electron microscope (TEM) and UV-visible spectroscopy. The antimicrobial effect of these different AgNPs against the gram negative bacterium Escherichia coli (E. coli) was studied by surface enhanced Raman spectroscopy (SERS), the evaluation of growth curves and inhibition zones. SERS proved to be sensitive to monitor the changes that occurred in the bacterial cells upon interaction with AgNPs, which qualitatively compared well with the data provided by the reference methods. However, as SERS is already sensitive to initial changes in the chemistry of bacteria due to the antibacterial effect of the AgNPs, fast and detailed information is provided by SERS as opposed to the classical reference methods based on the evaluation of growth curves and inhibition zones. The results of this work also demonstrate that hexagonal AgNPs display the highest antibacterial effect when compared to other NPs shapes, with triangular AgNPs exhibiting no antibacterial effect under the adopted conditions.
采用不同方法对不同形状的合成银纳米粒子（AgNPs）的抗菌效果进行了比较评价。通过透射电子显微镜（TEM）和紫外可见光谱法制备并表征了平均粒径为 40nm 的球形、三角形和六边形 AgNPs。通过表面增强拉曼光谱（SERS）、生长曲线和抑菌圈评价研究了这些不同 AgNPs 对革兰氏阴性菌大肠杆菌（E. coli）的抗菌效果。SERS 被证明可以灵敏地监测与 AgNPs 相互作用时细菌细胞发生的变化，这与参考方法提供的数据定性上非常吻合。然而，由于 SERS 对由于 AgNPs 的抗菌作用而导致的细菌化学初始变化已经很敏感，因此 SERS 提供了比基于生长曲线和抑菌圈评价的经典参考方法更快和更详细的信息。这项工作的结果还表明，与其他 NPs 形状相比，六方 AgNPs 显示出最高的抗菌效果，而在采用的条件下，三角形 AgNPs 没有抗菌效果。
