Tabari Mohaddeseh Abouhosseini, Youssefi Mohammad Reza, Nasiri Mojtaba, Hamidi Mojtaba, Kiani Komeil, Alian Samakkhah Shohre, Maggi Filippo
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol, Iran.
Vet Parasitol. 2019 Feb;266:84-87. doi: 10.1016/j.vetpar.2018.12.019. Epub 2019 Jan 17.
Cystic echinococcosis is of great public health importance. Surgery is the efficient treatment for this infection. To minimize the risk of cyst intraoperative leakage, using scolicidals is crucial. To date, any efficacious scolicidal without side effect has not been introduced. Since essential oils of Pelargonium roseum and Ferula gummosa have shown several bioactivities, we evaluated their potential against protoscoleces of E. granulosus using albendazole as positive control. Furthermore, chemical composition of both essential oils was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses, and their main constituents were also evaluated for scolicidal activity. Different concentrations of essential oils and their two main constituents were tested for scolicidal activity. Mortality rate was measured by eosin staining. Results of GC-MS revealed citronellol and β-pinene as the main constituents of P. roseum and F. gummosa essential oils, respectively. After 60 min of exposure to 50 μg/mL of P. roseum and F. gummosa, mean mortality rate of protoscoleces was 100%. However, β-pinene and citronellol at the same time point with only 10 μg/mL concentrations resulted in approximately higher than 80% mortality. Essential oils of P. roseum and F. gummosa showed significant toxic effect on E. granulosus with 50% lethal concentration (LC) values of 8.52 and 17.18 μg/mL, respectively. Based on the LC values, β-pinene (2.20 μg/mL) was the most potent scolicidal agent in the present study. The overall toxicity of β-pinene and citronellol was significantly higher than the whole essential oils of F. gummosa and P. roseum. Based on these results, β-pinene and citronellol can be considered as candidate ingredients for the development of green scolicidals.
囊型棘球蚴病对公共卫生具有重大意义。手术是治疗这种感染的有效方法。为了将囊肿术中渗漏的风险降至最低，使用杀头节剂至关重要。迄今为止，尚未引入任何无副作用的有效杀头节剂。由于香叶天竺葵和阿魏的精油已显示出多种生物活性，我们以阿苯达唑作为阳性对照，评估了它们对细粒棘球绦虫原头节的作用。此外，通过气相色谱 - 质谱（GC - MS）分析对两种精油的化学成分进行了分析，并对其主要成分的杀头节活性也进行了评估。测试了不同浓度的精油及其两种主要成分的杀头节活性。通过伊红染色测量死亡率。GC - MS结果显示，香茅醇和β - 蒎烯分别是香叶天竺葵和阿魏精油的主要成分。在暴露于50μg/mL的香叶天竺葵和阿魏精油60分钟后，原头节的平均死亡率为100%。然而，在同一时间点，仅10μg/mL浓度的β - 蒎烯和香茅醇导致的死亡率约高于80%。香叶天竺葵和阿魏的精油对细粒棘球绦虫显示出显著的毒性作用，其50%致死浓度（LC）值分别为8.52和17.18μg/mL。基于LC值，β - 蒎烯（2.20μg/mL）是本研究中最有效的杀头节剂。β - 蒎烯和香茅醇的总体毒性显著高于阿魏和香叶天竺葵的全精油。基于这些结果，β - 蒎烯和香茅醇可被视为开发绿色杀头节剂的候选成分。
