Li Jianyong, Chen Qian, Man Yahui, Pei Di, Wu Wenjian
Department of Chemistry and Biology, National University of Defense Technology, No. 109 Deya Road, Kaifu District, Changsha, Hunan, China.
Biochem Genet. 2019 Aug;57(4):571-582. doi: 10.1007/s10528-019-09910-8. Epub 2019 Feb 8.
Mosquitoes transmit many harmful diseases that seriously threaten public health. The mosquito's olfactory system is of great significance for host selection. Inotropic receptors (IRs) and olfactory receptors (ORs) have been demonstrated to be capable of odorant molecular recognition. Analyzing the molecular principles of mosquito olfaction facilitates the development of prevention and therapy techniques. Advances in the understanding of IRs have been seriously inadequate compared to those of ORs. Here, we provide evidence that 35 Anopheles sinensis IR (AsIR) genes are expressed, 7 of which are in the antennae and 2 have expression levels that are upregulated with a blood meal. A homologous analysis of the sequences showed that AsIRs are a subfamily of ionotropic glutamate receptors (iGLURs). This is the first that time IRs have been identified in Anopheles sinensis in vitro. The ultrastructure of the antennae supports the theory that diverse sensilla are distributed in the antennae. The results here may facilitate the revelation of the regulation mechanism in AsIRs, which could mitigate the transmission of diseases by mosquitoes.
蚊子传播许多有害疾病，严重威胁公众健康。蚊子的嗅觉系统对于宿主选择具有重要意义。亲离子受体（IRs）和嗅觉受体（ORs）已被证明能够识别气味分子。分析蚊子嗅觉的分子原理有助于预防和治疗技术的发展。与ORs相比，对IRs的理解进展严重不足。在此，我们提供证据表明，中华按蚊有35个IR（AsIR）基因表达，其中7个在触角中表达，2个的表达水平在取食血液后上调。序列同源性分析表明，AsIRs是亲离子型谷氨酸受体（iGLURs）的一个亚家族。这是首次在体外鉴定出中华按蚊中的IRs。触角的超微结构支持了不同感器分布在触角中的理论。这里的结果可能有助于揭示AsIRs的调控机制，从而减轻蚊子传播疾病的情况。
