Lee KathyAnn L, Marlow Florence L
Department of Cell Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Methods Mol Biol. 2019;1920:277-293. doi: 10.1007/978-1-4939-9009-2_16.
Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
本文描述了使用蛋白质、RNA和线粒体标记物来可视化斑马鱼初级卵母细胞的巴尔比亚尼小体的方法。该方法包括早期斑马鱼卵母细胞的分离、组织学、染色和显微镜检查。这些技术可用于可视化定位于巴尔比亚尼小体的基因产物，应用于突变体时，可用于解读早期卵母细胞中巴尔比亚尼小体发育过程中的分子和遗传途径。
