一名急性胸痛患者高敏心肌肌钙蛋白假阳性病例：干扰因素的分析研究

A false positive case of high-sensitivity cardiac troponin in a patient with acute chest pain: Analytical study of the interference.

作者信息

Baroni S, Troiani E, Santonocito C, Moretti G, De Luca C, Antenucci M, Urbani A

机构信息

UOC Chimica, Biochimica e Biologia Molecolare Clinica, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italy; Università Cattolica del Sacro Cuore, Roma, Italy.

Università Cattolica del Sacro Cuore, Roma, Italy.

出版信息

Clin Biochem. 2019 Apr;66:103-105. doi: 10.1016/j.clinbiochem.2019.02.002. Epub 2019 Feb 6.

DOI:10.1016/j.clinbiochem.2019.02.002

PMID:30738031

Abstract

We report a case of a heterophile antibodies interference in a new high-sensitivity troponin commercial immunoassay (cTNIH Siemens), observed in a patient with possible acute coronary syndrome (ACS). The analytical interference was investigated with standard laboratories procedures. The false positive result was found with different troponin methods and kits. We also investigated the protein sequence of cTnl and no sequence variants were detected. The discordance between clinical pictures and high concentration of cTnl, together with the collaboration between clinicians and laboratory staff avoided possible erroneous diagnosis and further invasive investigations to the patient.

摘要

我们报告了一例嗜异性抗体干扰新型高敏肌钙蛋白商业免疫测定法（西门子公司的cTNIH）的病例，该病例发生在一名可能患有急性冠状动脉综合征（ACS）的患者身上。采用标准实验室程序对分析干扰进行了研究。在不同的肌钙蛋白检测方法和试剂盒中均发现了假阳性结果。我们还研究了cTnl的蛋白质序列，未检测到序列变异。临床症状与高浓度cTnl之间的不一致，以及临床医生与实验室工作人员之间的合作，避免了对患者可能的错误诊断和进一步的侵入性检查。