Tan Peng, Dong Xiaojing, Xu Hanlin, Mai Kangsen, Ai Qinghui
Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of China.
Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, People's Republic of China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong, 266237, People's Republic of China.
Fish Shellfish Immunol. 2017 Apr;63:139-146. doi: 10.1016/j.fsi.2017.02.006. Epub 2017 Feb 8.
High percentage of dietary vegetable oil (VO) induced negative effects on immunity in numerous fish species. The present study was conducted to investigate whether VO could exert anti-immunological effects by regulating non-specific immunity, liver antioxidant capacity and nuclear factor kappa beta (NF-κB) signaling in Japanese sea bass (Lateolabrax japonicus). Three iso-nitrogenous and iso-lipid diets were formulated by replacing 0% (FO, the control), 50% (FV) and 100% (VO) of fish oil with vegetable oil. Each diet was randomly fed to triplicate groups of fish for 10 weeks. Results showed that the alternative complement pathway (ACP) activity and the disease resistance were significantly lower in fish fed VO diets compared with the control group (P < 0.05). Liver superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GPx) enzyme activities, as well as total antioxidant capacity (T-AOC) significantly decreased in fish fed VO diets (P < 0.05). Meanwhile, significantly low level of liver SOD1 and CAT mRNA, nuclear factor erythroid 2-related factor 2 (Nrf2) of both mRNA and protein were observed in fish fed VO diets when compared with fish fed FO diets (P < 0.05). However, the transcription level of TNFα and IL1β was significantly higher in the liver of fish fed VO diets, which might be attributed to the activation of NF-κB signaling pathway since the protein expression of p65, one of the key members of NF-κB family, was significantly increased (P < 0.05). These results suggested that dietary VO could lower the ACP activity, disease resistance and liver antioxidant capacity, but it could also exacerbate inflammatory response by activating NF-κB signaling pathway in Japanese sea bass.
高比例的膳食植物油（VO）对众多鱼类的免疫力产生负面影响。本研究旨在调查VO是否通过调节日本鲈鱼（Lateolabrax japonicus）的非特异性免疫、肝脏抗氧化能力和核因子κB（NF-κB）信号传导发挥抗免疫作用。通过用植物油替代0%（FO，对照组）、50%（FV）和100%（VO）的鱼油，配制了三种等氮和等脂的饲料。每种饲料随机投喂三组重复的鱼，持续10周。结果表明，与对照组相比，投喂VO饲料的鱼的替代补体途径（ACP）活性和抗病能力显著降低（P < 0.05）。投喂VO饲料的鱼的肝脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）酶活性以及总抗氧化能力（T-AOC）显著降低（P < 0.05）。同时，与投喂FO饲料的鱼相比，投喂VO饲料的鱼肝脏中SOD1和CAT mRNA、核因子红细胞2相关因子2（Nrf2）的mRNA和蛋白水平均显著降低（P < 0.05）。然而，投喂VO饲料的鱼肝脏中TNFα和IL1β的转录水平显著更高，这可能归因于NF-κB信号通路的激活，因为NF-κB家族的关键成员之一p65的蛋白表达显著增加（P < 0.05）。这些结果表明，膳食VO可降低日本鲈鱼的ACP活性、抗病能力和肝脏抗氧化能力，但也可通过激活NF-κB信号通路加剧炎症反应。
