Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems.
作者信息
Van Cott E M, Wilson G G
机构信息
New England Biolabs, Inc., Beverly, MA 01915.
出版信息
Gene. 1988 Dec 25;74(1):55-9. doi: 10.1016/0378-1119(88)90251-x.
PMID:3074019
Abstract
Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.
摘要
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