Putzu M, Causa F, Parente Manuel, González de Torre Israel, Rodriguez-Cabello J C, Netti P A
Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale (DICMAPI), University "Federico II", Piazzale Tecchio 80, Naples, Italy.
Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Piazzale Tecchio 80, Napoli, Italy.
Regen Biomater. 2019 Feb;6(1):21-28. doi: 10.1093/rb/rby022. Epub 2018 Oct 30.
In the field of tissue engineering the choice of materials is of great importance given the possibility to use biocompatible polymers produced by means of biotechnology. A large number of synthetic and natural materials have been used to this purpose and processed into scaffolds using Electrospinning technique. Among materials that could be used for the fabrication of scaffold and degradable membranes, natural polymers such as collagen, elastin or fibroin offer the possibility to design structures strictly similar to the extracellular matrix (ECM). Biotechnology and genetic engineering made possible the advent of a new class of biopolymers called protein-based polymers. One example is represented by the silk-elastin-proteins that combine the elasticity and resilience of elastin with the high tensile strength of silk-fibroin and display engineered bioactive sequences. In this work, we use electrospinning technique to produce a fibrous scaffold made of the co-recombinamer Silk-ELR. Obtained fibres have been characterized from the morphological point of view. Homogeneity and morphology have been explored using Scanning Electron Microscopy. A thorough study regarding the influence of Voltage, flow rate and distance have been carried out to determine the appropriate parameters to obtain the fibrous mats without defects and with a good distribution of diameters. Cytocompatibility has also been tested. For the first time we use the co-recombinamer Silk-ELR for the fabrication of a 2.5 angioplasty balloon coating. This structure could be useful as a coated scaffold for the regeneration of intima layer of vessels.
在组织工程领域，鉴于有可能使用通过生物技术生产的生物相容性聚合物，材料的选择至关重要。大量的合成材料和天然材料已被用于此目的，并使用静电纺丝技术加工成支架。在可用于制造支架和可降解膜的材料中，天然聚合物如胶原蛋白、弹性蛋白或丝素蛋白提供了设计与细胞外基质（ECM）严格相似结构的可能性。生物技术和基因工程使得一类新型生物聚合物——基于蛋白质的聚合物得以出现。一个例子是以丝素蛋白 - 弹性蛋白 - 蛋白质为代表，它将弹性蛋白的弹性和回弹性与丝素蛋白的高拉伸强度相结合，并展示出工程化的生物活性序列。在这项工作中，我们使用静电纺丝技术生产由共重组体丝素蛋白 - 弹性蛋白 - 赖氨酸 - 精氨酸（Silk - ELR）制成的纤维支架。从形态学角度对所得纤维进行了表征。使用扫描电子显微镜探索了其均匀性和形态。对电压、流速和距离的影响进行了深入研究，以确定获得无缺陷且直径分布良好的纤维垫的合适参数。还测试了细胞相容性。我们首次使用共重组体丝素蛋白 - 弹性蛋白 - 赖氨酸 - 精氨酸（Silk - ELR）制造2.5血管成形术球囊涂层。这种结构可作为血管内膜层再生的涂层支架。
