Wilkins E S, Wilkins M G
Department of Chemical and Nuclear Engineering, Farris Engineering Center, University of New Mexico, Albuquerque.
Horm Metab Res Suppl. 1988;20:50-5.
Previous techniques for sensing and measuring glucose are briefly reviewed and compared. The genesis of the glucose coated wire electrode lies in liquid membrane technology, and the basic principle of the electrode is described. The electrode utilizes a quaternary ammonium salt with a sparingly soluble metallic salt of glucose, in a matrix such as poly(vinyl chloride). Fabrication procedures are detailed. Typical sensor responses are illustrated with experimental data. The sensor generally shows a voltage that decreases linearly with increasing glucose concentration over approximately 40 mg/dl to 200 mg/dl. The time response of the sensor is of the order of seconds, and the lifetime observed to date is of the order of several months. Initial studies of mechanism are discussed, using liquid NMR. Preliminary interference effect studies to date are summarized, in which common compounds such as ascorbic acid, uric acid, L-cystine, glycine, and bilirubin were used. Singly these compounds show no significant interference effects.
本文简要回顾并比较了以往用于检测和测量葡萄糖的技术。葡萄糖包被丝电极起源于液膜技术，并描述了该电极的基本原理。该电极在聚氯乙烯等基质中使用季铵盐与难溶性葡萄糖金属盐。详细介绍了制备过程。用实验数据说明了典型的传感器响应。该传感器通常显示出一种电压，在大约40mg/dl至200mg/dl的葡萄糖浓度范围内，电压随葡萄糖浓度的增加而线性下降。传感器的时间响应为秒级，迄今为止观察到的寿命为几个月左右。讨论了使用液体核磁共振进行的初步机理研究。总结了迄今为止的初步干扰效应研究，其中使用了抗坏血酸、尿酸、L-胱氨酸、甘氨酸和胆红素等常见化合物。单独使用这些化合物时，没有显示出明显的干扰效应。
