Technology Transfer Center, Kunming Medical University, Kunming 650500, China; Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, China.
Brain Res. 2019 Jun 1;1712:207-216. doi: 10.1016/j.brainres.2019.02.006. Epub 2019 Feb 8.
Brain damage, including blood-brain barrier (BBB) dysfunction, neurological behavior deficit, cerebral infarction and inflammation, is commonly caused by ischemic-reperfusion (I/R) injury. Prevention of the above biological process defects is considered beneficial for patient recovery after I/R injury. This study was aimed to assess the neuroprotective effect of Gastrodin (GAS), an herbal agent, in experimentally induced cerebral ischemia. Sprague-Dawley adult rats were randomly divided into six groups: Sham-operated control group (Sham), middle cerebral artery occlusion (MCAO) group, GAS (50, 100, and 200 mg/kg) pretreatment + MCAO groups (GAS) and Nimodipine (NIM) + MCAO, namely, the NIM group. Additionally, an OGD/R model using BV-2 microglia was established in vitro to simulate I/R injury. We showed here that the neurological scores of rats in the GAS groups were significantly improved compared with the MCAO group. Moreover, the area of cerebral infarction in the GAS pretreatment groups and the NIM group was significantly reduced. Furthermore, Evans blue leakage volume was significantly reduced with GAS pretreatment notably at dose 100 mg/kg. Expression of matrix metalloproteinase 2 (MMP2) and MMP9 in GAS groups was markedly decreased when compared with MCAO group. In BV-2 microglia exposed to OGD/R given GAS pretreatment, MMP2 and MMP9 positive cells were reduced in numbers. The present results have shown that GAS pretreatment significantly compensated for neurological behavior defects in rats with I/R-induced injury, reduced brain infarction size, reversed BBB impairment, and attenuated inflammation. It is suggested that pretreatment with GAS before surgery is beneficial during recovery from I/R injury.
脑损伤，包括血脑屏障（BBB）功能障碍、神经行为缺陷、脑梗死和炎症，通常是由缺血再灌注（I/R）损伤引起的。预防上述生物过程缺陷被认为有利于 I/R 损伤后患者的恢复。本研究旨在评估天麻素（GAS）这种草药制剂对实验性脑缺血的神经保护作用。成年 Sprague-Dawley 大鼠随机分为六组：假手术对照组（Sham）、大脑中动脉闭塞组（MCAO）、天麻素（50、100 和 200mg/kg）预处理+MCAO 组（GAS）和尼莫地平+MCAO 组（NIM）。此外，还建立了体外氧葡萄糖剥夺/复氧（OGD/R）模型来模拟 I/R 损伤。我们发现，GAS 组大鼠的神经评分明显优于 MCAO 组。此外，GAS 预处理组和 NIM 组的脑梗死面积明显减少。此外，GAS 预处理可明显减少 Evans 蓝漏出量，尤以 100mg/kg 剂量作用显著。与 MCAO 组相比，GAS 组基质金属蛋白酶 2（MMP2）和 MMP9 的表达明显降低。在给予 GAS 预处理的 OGD/R 暴露的 BV-2 小胶质细胞中，MMP2 和 MMP9 阳性细胞数量减少。这些结果表明，天麻素预处理可显著补偿 I/R 损伤大鼠的神经行为缺陷，减少脑梗死面积，逆转 BBB 损伤，并减轻炎症。提示术前给予天麻素预处理有利于 I/R 损伤后的恢复。
