Viral Immunology and Intravital Imaging Section, National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
Bio-Rad Laboratories, Hercules, CA 94547, USA.
Methods. 2019 Apr 1;158:22-26. doi: 10.1016/j.ymeth.2019.02.007. Epub 2019 Feb 10.
This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.
本方案描述了如何使用基于多重微珠的免疫分析试剂盒从鼠脑组织中定量检测多种炎症介质。从少量组织中定量检测多种分析物的方法非常重要。Bio-Plex 是一种基于 Luminex xMAP 的多重微珠免疫分析技术，可从单个组织样本中同时分析多达 100 种分析物。该检测法已广泛用于分析血浆和血清样本以及培养的和原代细胞中的分析物。在此，我们描述了一种使用 Bio-Plex Pro Mouse Chemokine Panel 33-Plex 从鼠脑组织中同时分析 33 种不同炎症细胞因子和趋化因子的方法。
