Harris Greg, Chen Wangxue
Human Health and Therapeutics Research Center, National Research Council of Canada, Ottawa, ON, Canada.
Methods Mol Biol. 2019;2024:79-94. doi: 10.1007/978-1-4939-9597-4_4.
Multiplex bead array technology expands upon the principles of the enzyme-linked immunosorbent assay by allowing the simultaneous quantification of a large number of cytokines and chemokines within a single sample. This allows researchers more freedom and opportunities to investigate complex immune responses both in vivo and in vitro. Here we describe and update the detailed assay protocol and technical tips for simultaneous quantification of multiple cytokines and chemokines in mouse biological fluids such as sera, bronchoalveolar lavage fluid, tissue homogenate supernatant, and tissue culture supernatant, using a multiplex bead array assay.
多重微珠阵列技术在酶联免疫吸附测定原理的基础上进行了扩展，它能够在单个样本中同时对大量细胞因子和趋化因子进行定量分析。这使研究人员在体内和体外研究复杂免疫反应时有了更多的自由和机会。在此，我们描述并更新了详细的检测方案和技术提示，用于使用多重微珠阵列分析法同时定量小鼠生物体液（如血清、支气管肺泡灌洗液、组织匀浆上清液和组织培养上清液）中的多种细胞因子和趋化因子。
