Lu Shi-En, Gross Dennis C
Department of Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762.
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843-2132.
Plant Dis. 2010 Dec;94(12):1431-1440. doi: 10.1094/PDIS-05-10-0365.
Drippy pod is a unique bacterial disease of Mediterranean white lupine (Lupinus albus) that first appeared in commercial fields in Eastern Washington State in the mid-1980s. The disease is most noticeable in the field as water-soaked lesions on lupine pods that produce an abundance of whitish-colored ooze with a sticky and foamy consistency. As the disease progresses, yellowing of lupine plants occurs with ooze characteristically dripping down the infected pods and stems and solidifying. A gram-negative rod-shaped bacterium with facultative anaerobic growth was repeatedly isolated from infected lupine tissues, and subsequently confirmed by Koch's postulates to infect lupines. Physiological and biochemical tests, including the API 20E and 50CHE strip assays, showed a highly uniform phenotype for the lupine strains that was distinctive for the genus Brenneria and most closely resembled the oak pathogen Brenneria quercina. Furthermore, sequence analyses of the 16S rDNA gene and the 16S-23S intergenic region of lupine strains revealed the highest similarity (>97%) to the corresponding regions of B. quercina and less similarity to the next closest species, B. salicis. Fatty acid profiling demonstrated that lupine strains were qualitatively similar in composition to Brenneria spp., and supported placement of the drippy pod bacterium in the species B. quercina. Oak strains of B. quercina, however, did not incite drippy pod disease on lupine. Consequently, the lupine strains that cause bacterial drippy pod disease were classified as B. quercina pv. lupinicola pv. nov.
滴漏荚病是地中海白羽扇豆（羽扇豆）特有的一种细菌性病害，于20世纪80年代中期首次出现在华盛顿州东部的商业种植田中。在田间，这种病害最明显的表现是羽扇豆荚上出现水渍状病斑，病斑会产生大量白色、具有粘性和泡沫状的渗出物。随着病情发展，羽扇豆植株会发黄，渗出物会顺着受感染的豆荚和茎滴下并凝固。从受感染的羽扇豆组织中反复分离出一种兼性厌氧生长的革兰氏阴性杆状细菌，随后通过柯赫氏法则证实其可感染羽扇豆。包括API 20E和50CHE条带检测在内的生理生化测试表明，羽扇豆菌株具有高度一致的表型，这在布伦纳氏菌属中是独特的，且与橡树病原菌栎布伦纳氏菌最为相似。此外，对羽扇豆菌株的16S rDNA基因和16S - 23S基因间隔区的序列分析显示，其与栎布伦纳氏菌相应区域的相似度最高（>97%），与次近缘物种柳树布伦纳氏菌的相似度较低。脂肪酸谱分析表明，羽扇豆菌株在组成上与布伦纳氏菌属定性相似，支持将滴漏荚病菌归类为栎布伦纳氏菌羽扇豆致病变种（新变种）。然而，栎布伦纳氏菌的橡树菌株不会在羽扇豆上引发滴漏荚病。因此，引起细菌性滴漏荚病的羽扇豆菌株被归类为栎布伦纳氏菌羽扇豆致病变种（新变种）。
