Jeonju AgroBio-Materials Institute, 54810, Wonjangdong-gil 111-27, Deokjin-gu, Jeonju-si 54810, Jeollabuk-do, Korea.
Nutrients. 2019 Feb 8;11(2):356. doi: 10.3390/nu11020356.
(L.) Britt. var. japonica (Hassk.) Hara (PF), is a medical herb of the Lamiaceae family. We have previously reported that the PF sprout extract (PFSE) is effective in treating hyperglycemia. However, the role of PFSE on glomerular mesangial cells (MCs) proliferation and the extracellular matrix (ECM) accumulation in a diabetic condition are still unclear. Therefore, in this study, we have investigated the role of PFSE on cell proliferation and ECM accumulation in murine glomerular MCs (MMCs), cultured under a high glucose (HG) condition. PFSE treatment attenuated HG-induced MMCs proliferation and hypertrophy. Moreover, the HG-induced ECM protein, collagen IV and fibronectin, overexpression was abolished by the PFFSE treatment. In addition, PFSE inhibited reactive oxygen species (ROS) overproduction and NOX2 and NOX4 expression in MMCs under a HG condition. Our data further revealed the involvement of mesangial cell damage in AMP-activated kinase (AMPK) activation. PFSE strongly activated AMPK in MMCs under hyperglycemic conditions. These results suggest that PFSE inhibits HG-medicated MC fibrosis through suppressing the activation of NOX2/4 and the AMPK activation mechanism. PFSE may be useful for the prevention or treatment of diabetic nephropathy.
（L.） Britt. var. japonica (Hassk.) Hara (PF) 是唇形科的一种药用草本植物。我们之前曾报道过 PF 芽提取物（PFSE）在治疗高血糖方面有效。然而，PFSE 对糖尿病状态下肾小球系膜细胞（MCs）增殖和细胞外基质（ECM）积累的作用尚不清楚。因此，在这项研究中，我们研究了 PFSE 在高葡萄糖（HG）条件下培养的鼠肾小球 MCs（MMCs）中的细胞增殖和 ECM 积累中的作用。PFSE 处理可减轻 HG 诱导的 MMCs 增殖和肥大。此外，PFSE 处理可消除 HG 诱导的 ECM 蛋白、胶原 IV 和纤连蛋白的过度表达。此外，PFSE 可抑制 HG 条件下 MMCs 中超氧阴离子（ROS）的过度产生和 NOX2 和 NOX4 的表达。我们的数据进一步揭示了系膜细胞损伤在 AMP 激活的蛋白激酶（AMPK）激活中的作用。PFSE 在高血糖条件下可强烈激活 MMC 中的 AMPK。这些结果表明，PFSE 通过抑制 NOX2/4 的激活和 AMPK 激活机制来抑制 HG 介导的 MC 纤维化。PFSE 可能有助于预防或治疗糖尿病肾病。
