Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
Food Chem. 2019 Jun 30;284:279-286. doi: 10.1016/j.foodchem.2019.01.071. Epub 2019 Jan 19.
Benzimidazoles (BZDs) are widely used veterinary drugs in the domestic food-producing animals, resulting in the metabolism of BZDs and the harmful metabolites residues in some foods. However, some BZD metabolites are unknown and so it is important to discover new metabolites to expand detection targets. For these reasons, a sensitive and selective strategy was designed to identify BZD metabolites in the serum of pigs after oral administration of albendazole, fenbendazole and thiabendazole, respectively. Nickel oxide nanoparticle-deposited silica (SiO@NiO) composite was used for the enrichment and purification of BZD compounds. High-performance liquid chromatography coupled with precursor ion scan-mass spectrometry (LC-PIS-MS) and high resolution MS/MS analysis (HR-MS/MS) was employed for the BZDs metabolic profiles characterization and metabolites detection. Finally, 18 BZD metabolites were identified, among which 11 metabolites were discovered in pig serum for the first time. Besides, more comprehensive BZD metabolic pathways were presented.
苯并咪唑类药物（BZDs）是广泛应用于国内食用动物的兽药，导致 BZD 的代谢物和一些食物中的有害代谢物残留。然而，一些 BZD 代谢物是未知的，因此发现新的代谢物以扩大检测目标非常重要。基于这些原因，设计了一种灵敏和选择性的策略，以分别鉴定口服阿苯达唑、芬苯达唑和噻苯达唑后猪血清中的 BZD 代谢物。使用氧化镍纳米颗粒沉积的二氧化硅（SiO@NiO）复合材料进行 BZD 化合物的富集和纯化。采用高效液相色谱-前体离子扫描质谱（LC-PIS-MS）和高分辨 MS/MS 分析（HR-MS/MS）进行 BZDs 代谢谱特征描述和代谢物检测。最后，鉴定了 18 种 BZD 代谢物，其中 11 种代谢物是首次在猪血清中发现的。此外，还呈现了更全面的 BZD 代谢途径。
