Kardan M, Rafiei A, Ghaffari J, Valadan R, Morsaljahan Z, Haj-Ghorbani S T
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Molecular and Cell Biology Center, Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Allergol Immunopathol (Madr). 2019 Jul-Aug;47(4):378-385. doi: 10.1016/j.aller.2018.12.003. Epub 2019 Feb 10.
Allergic asthma is a chronic inflammatory disorder of the airways. Th1, Th2 and Th17 cells are the main cells involved in the pathophysiology of asthma. The function of these cells is affected by T-bet, GATA3 and RORγt transcription factors (respectively). Therefore, the aim of this study was to evaluate the effect of ginger (officinal Roscoe) extract on the expression of T-bet, GATA-3 and ROR-γ in peripheral blood mononuclear cells (PBMC) of asthmatic patients, in comparison with healthy volunteers as controls.
In this case-control study, a total of 50 individuals including 25 patients with severe, moderate and mild allergic asthma and 25 unrelated healthy controls were involved. The PBMCs were isolated and divided into four groups: negative control, two positive controls (Budesonide and PHA) and ginger-extract treated group. After cell treatment and incubation for 48h, PBMCs were isolated and cDNA was synthesized. Gene expressions of T-bet, GATA3 and ROR-γt were evaluated by Real-time PCR.
According to the results of this study, hydroalcoholic extract of ginger could reduce the expression of GATA-3, ROR-γt, and T-bet in PBMCs of asthmatic patients in comparison with untreated PBMCs (P values=0.001, 0.001, and 0.002, respectively). It was also shown that the ginger extract could affect T-bet/GATA-3, T-bet/ROR-γt, and ROR-γt/GATA-3 expression ratios.
This study showed that the use of ginger extract could control asthma and decrease the severity of this disease by affecting the main cells involving the symptoms of asthma in the airways.
过敏性哮喘是一种气道慢性炎症性疾病。Th1、Th2和Th17细胞是参与哮喘病理生理学的主要细胞。这些细胞的功能分别受T-bet、GATA3和RORγt转录因子影响。因此，本研究旨在评估姜（药用姜）提取物对哮喘患者外周血单个核细胞（PBMC）中T-bet、GATA-3和ROR-γ表达的影响，并与健康志愿者作为对照进行比较。
在这项病例对照研究中，共纳入50名个体，包括25例重度、中度和轻度过敏性哮喘患者以及25名无亲缘关系的健康对照。分离PBMC并分为四组：阴性对照、两个阳性对照（布地奈德和PHA）和姜提取物处理组。细胞处理并孵育48小时后，分离PBMC并合成cDNA。通过实时PCR评估T-bet、GATA3和ROR-γt的基因表达。
根据本研究结果，与未处理的PBMC相比，姜的水醇提取物可降低哮喘患者PBMC中GATA-3、ROR-γt和T-bet的表达（P值分别为0.001、0.001和0.002）。还表明姜提取物可影响T-bet/GATA-3、T-bet/ROR-γt和ROR-γt/GATA-3的表达比率。
本研究表明，使用姜提取物可通过影响气道中涉及哮喘症状的主要细胞来控制哮喘并降低疾病严重程度。
