Institute of Cell Biology - Heussler Laboratory, University of Bern, Bern, Switzerland; Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, Scotland, UK; Current affiliation: Instituto de Medicina Molecular - João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal; These authors contributed equally.
Institute of Applied Physics, University of Bern, Bern, Switzerland; These authors contributed equally.
Trends Parasitol. 2019 Mar;35(3):193-212. doi: 10.1016/j.pt.2019.01.002. Epub 2019 Feb 10.
Animal models have for long been pivotal for parasitology research. Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host-pathogen interactions by allowing different visualization forms in vivo and ex vivo. Advances including increased resolution, penetration depth, and acquisition speed, together with more complex image analysis methods, facilitate tackling biological problems previously impossible to study and/or quantify. Here we discuss advances and challenges in the in vivo imaging toolbox, which hold promising potential for the field of parasitology.
动物模型长期以来一直是寄生虫学研究的关键。在过去的几年中，活体、光声和磁共振成像、光学投影断层扫描和选择性平面照明显微镜等技术发展出了有前景的潜力，可以通过允许不同的可视化形式在体内和体外来深入了解宿主-病原体相互作用。包括提高分辨率、穿透深度和采集速度在内的进展，以及更复杂的图像分析方法，有助于解决以前无法研究和/或量化的生物学问题。在这里，我们讨论了体内成像工具包的进展和挑战，该工具包为寄生虫学领域带来了有前途的潜力。
