BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Bioengineering, KRIBB School, University of Science and Technology (UST), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Biosens Bioelectron. 2019 Apr 1;130:225-229. doi: 10.1016/j.bios.2019.01.033. Epub 2019 Jan 21.
This study demonstrates a novel and rapid antibacterial susceptibility testing (AST) method, called fluorescence resonance energy transfer (FRET) probe-based AST (F-AST), which relies on a nuclease-activated FRET probe that detects bacterial nucleases released by antibiotic-induced bacterial lysis. Three quality control (QC) strains and two additional clinically important strains were tested, and the minimum inhibitory concentration (MIC) values from both gold standard AST method (broth microdilution (BMD)) and the new F-AST method were compared. The resulting fluorescence signals from the F-AST method were obtained within 3-6 h and were consistent with MIC values obtained from the BMD method, which took more than 16 h. Thus, the F-AST method is a simple and rapid tool to detect antibacterial susceptibility, including MIC values, and provides a basis for rapid clinical treatments.
本研究展示了一种新颖而快速的抗菌药敏测试（AST）方法，称为荧光共振能量转移（FRET）探针 AST（F-AST），它依赖于一种核酸酶激活的 FRET 探针，用于检测抗生素诱导的细菌裂解释放的细菌核酸酶。本研究测试了三种质控（QC）菌株和另外两种临床重要菌株，并比较了金标准 AST 方法（肉汤微量稀释法（BMD））和新的 F-AST 方法的最小抑菌浓度（MIC）值。F-AST 方法产生的荧光信号可在 3-6 小时内获得，与 BMD 方法获得的 MIC 值一致，BMD 方法耗时超过 16 小时。因此，F-AST 方法是一种简单快速的检测抗菌药敏性（包括 MIC 值）的工具，为快速临床治疗提供了依据。
