Department of Preclinical Conservative Dentistry and Preclinical Endodontics, University of Medical Sciences in Poznan, Poland.
Department of Medical Microbiology, University of Medical Sciences in Poznan, Poland.
Int J Med Sci. 2019 Jan 1;16(2):241-246. doi: 10.7150/ijms.29027. eCollection 2019.
: Data currently available indicate the significance of salivary mucins MUC5B and MUC7 in the protection of teeth against caries. Our study aimed to determine the relationship between dental caries in adults and levels of MUC5B and MUC7. : The studies were conducted on 45 adult subjects selected on the basis of dental examination and calculation of the DMFT (Decayed, Missing, and Filled Teeth) index. Among these patients, two research groups were distinguished: group 1 included 19 caries-free subjects (DMFT = 0); and group 2 included 26 patients with severe caries (DMFT > 13.9). Samples of whole unstimulated saliva were collected and centrifuged. MUC5B and MUC7 content in saliva supernatant were estimated using an enzyme-linked immunosorbent sandwich assay (ELISA). Analysis of the obtained data receiver operating characteristic (ROC) curves was employed to define relationships between the contents of the studied mucins and the detected dental caries. : In subjects from group 1, the mean level of MUC5B amounted to 0.63 ± 0.35 ng/ml and this was significantly higher than the concentration of mucin in patients of group 2, which amounted to 0.38 ± 0.32 ng/ml ( = 0.023). The mean level of MUC7 amounted to 5.47 ± 1.18 ng/ml and this was significantly higher than the level of the mucin in group 2, which was 1.39 ± 0.86 ng/ml (< 0.0001). In parallel, a relationship was detected between levels of the examined mucins and manifestation of dental caries. For MUC7, the optimal cut-off value was obtained (i.e. corresponding to 100% sensitivity and specificity), amounting to 2.5 ng/ml for the detection of dental caries risk. : Development of dental caries is linked to reduced concentrations of MUC5B and MUC7. The level of MUC7 may represent a significant parameter clinically suitable for evaluation of disease risk.
: 现有数据表明唾液黏蛋白 MUC5B 和 MUC7 在牙齿龋病保护中的重要性。本研究旨在确定成人龋齿与 MUC5B 和 MUC7 水平之间的关系。 : 本研究共纳入 45 名成年受试者，根据口腔检查和 DMFT（龋失补牙数）指数计算进行选择。这些患者分为两组：组 1 为 19 名无龋患者（DMFT = 0）；组 2 为 26 名患有严重龋齿患者（DMFT > 13.9）。采集全唾液样本并离心。采用酶联免疫吸附试验（ELISA）测定唾液上清液中 MUC5B 和 MUC7 的含量。采用受试者工作特征（ROC）曲线分析获得的数据，以确定研究中黏蛋白的含量与检测到的龋齿之间的关系。 : 在组 1 受试者中，MUC5B 的平均水平为 0.63 ± 0.35ng/ml，显著高于组 2 患者的浓度（0.38 ± 0.32ng/ml， = 0.023）。MUC7 的平均水平为 5.47 ± 1.18ng/ml，显著高于组 2 的水平（1.39 ± 0.86ng/ml，< 0.0001）。同时，还检测到所研究的黏蛋白水平与龋齿表现之间存在相关性。对于 MUC7，获得了最佳截断值（即 100%的敏感性和特异性），检测龋齿风险的截断值为 2.5ng/ml。 : 龋齿的发生与 MUC5B 和 MUC7 浓度降低有关。MUC7 水平可能是一种有意义的临床参数，适用于评估疾病风险。
