Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Hunan Provincial Engineering Research Center for Ginkgo biloba, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425100, China.
Int J Biol Sci. 2019 Jan 1;15(2):416-429. doi: 10.7150/ijbs.26884. eCollection 2019.
To obtain insight into the function of miRNAs in the synthesis and storage of important nutrients during the development of fruit, Illumina sequencing of flower and fruit small-RNA was conducted. The results revealed that 797 miRNAs were significantly differentially expressed between flower and fruit samples of . Through integrated GO and KEGG function annotations, it was determined that the miRNA target genes were mainly involved in metabolic pathways, plant hormone signal transduction, fruit development, mitosis and regulation of biosynthetic processes. Carbohydrate accumulation genes were differentially regulated by miR156, miR390 and miR395 in the fruit growth and development process. MiR477 is the key miRNA functioning in regulation of genes and involved in fatty acid synthesis. Additionally, miR156 also has the function of regulating glycolysis and nutrient transformation genes.
为了深入了解 miRNA 在果实发育过程中重要营养物质的合成和储存中的功能，对花和果实小 RNA 进行了 Illumina 测序。结果表明， 花和果实样本之间有 797 个 miRNA 显著差异表达。通过综合的 GO 和 KEGG 功能注释，确定 miRNA 靶基因主要参与代谢途径、植物激素信号转导、果实发育、有丝分裂和生物合成过程的调节。miR156、miR390 和 miR395 在果实生长发育过程中对碳水化合物积累基因进行差异调控。miR477 是调控基因和参与脂肪酸合成的关键 miRNA。此外，miR156 还具有调节糖酵解和营养转化基因的功能。
