Min Jinsoo, Kim Kyungjong, Choi Hongjo, Kang Eun Seok, Shin Yoon Mi, An Jin Young, Choe Kang Hyeon, Lee Ki Man
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Daejeon St. Mary's Hospital, The Catholic University of Korea, Daejeon, Republic of Korea.
Department of Research and Development, Korean Institute of Tuberculosis, Osong, Cheongju, Republic of Korea.
Ann Thorac Med. 2019 Jan-Mar;14(1):90-93. doi: 10.4103/atm.ATM_184_18.
Although accurate identification of is the gold standard for tuberculosis (TB) diagnosis, there have been several reports of false-positive results. After identifying a sudden increase in extensively drug-resistant TB, false-positive mycobacterial culture tests were suspected, and we contacted the supranational reference center for molecular typing. genotyping tests showed that isolates from all five patients had an identical genotype pattern, and all harbored the same Beijing strain based on sequence-based phylogenic analysis and drug-resistant single nucleotide polymorphisms (SNPs) analysis. We also used whole genome sequencing (WGS) to compare the SNPs of all isolates with a reference genome, and all were identical. We adapted WGS to efficiently detect false-positive MTB culture tests.
虽然准确鉴定[具体内容缺失]是结核病（TB）诊断的金标准，但已有数例假阳性结果的报告。在发现广泛耐药结核病突然增加后，怀疑分枝杆菌培养试验出现假阳性，我们联系了超国家分子分型参考中心。[具体内容缺失]基因分型测试表明，所有五名患者的分离株具有相同的基因型模式，基于序列系统发育分析和耐药单核苷酸多态性（SNP）分析，所有分离株均携带相同的北京菌株。我们还使用全基因组测序（WGS）将所有分离株的SNP与参考基因组进行比较，结果均相同。我们采用WGS来有效检测结核分枝杆菌培养试验的假阳性。
