Scott D W, Layton J E, Johnson G R
Eur J Immunol. 1978 Apr;8(4):286-8. doi: 10.1002/eji.1830080413.
Separation of IgD-bearing B lymphocytes from the spleens of 3-week-old or adult mice has been achieved using cellular affinity columns and an anti-delta allotype serum. The results demonstrate that the majority of cells capable of forming B lymphocyte colonies in agar are delta-positive although they lose detectable IgD upon stimulation. It is proposed that these cells are the descendants of IgD-negative cells found in the fetal liver and neonatal spleen.
利用细胞亲和柱和抗δ同种异型血清，已成功从3周龄或成年小鼠的脾脏中分离出携带IgD的B淋巴细胞。结果表明，尽管在刺激后它们失去了可检测到的IgD，但大多数能够在琼脂中形成B淋巴细胞集落的细胞是δ阳性的。有人提出，这些细胞是在胎儿肝脏和新生脾脏中发现的IgD阴性细胞的后代。
