Pifer L L, Woods D, Hughes W T
Infect Immun. 1978 Apr;20(1):66-8. doi: 10.1128/iai.20.1.66-68.1978.
Pneumocystis carinii derived from infected murine lung was found to be capable of limited growth in Vero African green monkey kidney cell cultures. The observed increase in the number of cyst forms was influenced by the ratio of cysts to host cells in the inocula, duration of passage, and formulation of the cell culture media. Maximum growth was achieved by inoculating 1.3 X 10(5) cysts per 75-cm2 flask containing about 2 X 10(7) Vero cells (cyst-to-cell ratio=1:154) maintained on minimal essential medium supplemented with 2% fetal bovine serum. Under these conditions of culture, a 10.8-fold increase in cyst forms was observed during a 7-day passage in cell culture utilizing cyst-to-cell ratios ranging from 1:28 to 1:2,778.
从受感染的鼠肺中分离出的卡氏肺孢子虫被发现能够在非洲绿猴肾细胞（Vero细胞）培养物中进行有限生长。观察到的包囊形式数量的增加受到接种物中包囊与宿主细胞的比例、传代时间以及细胞培养基配方的影响。通过在每75平方厘米培养瓶中接种1.3×10⁵个包囊，该培养瓶中含有约2×10⁷个Vero细胞（包囊与细胞比例为1:154），并维持在添加2%胎牛血清的最低必需培养基上，可实现最大生长。在这些培养条件下，利用包囊与细胞比例范围从1:28到1:2778的细胞培养物，在7天传代过程中观察到包囊形式增加了10.8倍。
