Department of Entomology, The Ohio State University, Wooster, OH.
The Center for Applied Plant Sciences, The Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH.
J Econ Entomol. 2019 May 22;112(3):1403-1410. doi: 10.1093/jee/toz007.
Cytochrome (CYP) P450s are a superfamily of enzymes that detoxify xenobiotics and regulate numerous physiological processes in insects. The genomes of phytophagous insects usually contain large numbers of P450s, especially within the CYP3 clan. Within this clan, CYP6 subfamily members help detoxify plant host secondary metabolites. In this study, we analyzed three CYP6 genes in the highly polyphagous invasive pest, Halyomorpha halys (Stål), commonly known as brown marmorated stink bug. We characterized and validated the expression of HhCYP6BQ27, HhCYP6BK13, and HhCYP6BK24 among sexes, tissues (gut, fat body, and Malpighian tubules) and hosts (apple, corn, soybean). Sequence characterization by amino acid alignments confirmed the presence of conserved motifs typical of the P450 superfamily. No significant differences existed in gene expression among sexes or when fed different hosts, suggesting that these transcripts might have broad substrate specificities. However, significant differences in gene expression were observed among the tissues studied and were gene-dependent. Collectively, the results show that H. halys differentially expressed CYP6 genes among tissues, which may be related to important and specific physiological functions. This study has increased our understanding of H. halys biology that can be useful for functional studies and can potentially be exploited in developing sustainable pest management strategies.
细胞色素（CYP）P450 超家族是一组能够解毒外来物质并调节昆虫体内众多生理过程的酶。植食性昆虫的基因组通常包含大量的 P450 基因，尤其是在 CYP3 家族内。在这个家族中，CYP6 亚家族成员有助于解毒植物宿主的次生代谢物。在这项研究中，我们分析了高度多食性入侵害虫褐带长蝽（Halyomorpha halys）（Stål）中的三个 CYP6 基因，该害虫通常被称为棕带斑蝥。我们对 HhCYP6BQ27、HhCYP6BK13 和 HhCYP6BK24 在雌雄两性、组织（肠道、脂肪体和马氏管）和宿主（苹果、玉米、大豆）之间的表达进行了特征描述和验证。通过氨基酸比对进行的序列特征分析证实了存在典型的 P450 超家族保守基序。雌雄两性之间或在不同宿主上进食时，基因表达没有显著差异，这表明这些转录物可能具有广泛的底物特异性。然而，在所研究的组织之间观察到基因表达存在显著差异，并且这种差异是基因依赖性的。总的来说，这些结果表明，褐带长蝽在组织之间差异表达 CYP6 基因，这可能与重要和特定的生理功能有关。本研究增加了我们对褐带长蝽生物学的理解，这对于功能研究可能是有用的，并可能被用于开发可持续的害虫管理策略。
