Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
J Anim Sci. 2019 Apr 29;97(5):2114-2124. doi: 10.1093/jas/skz038.
This study was to investigate the effect of zinc pectin oligosaccharides chelate (Zn-POS) on growth performance, serum enzyme activities, tissue zinc accumulation, metallothionein (MT) concentrations, and gene expression of zinc transporters (ZnT) in broilers. Five hundred forty 1-d-old Arbor Acres broiler chicks were randomly assigned to 5 dietary groups with 6 replicates of 18 birds per replicate. The diets were formulated with the same supplemental Zn level (80 mg/kg diet) but different amount of the Zn-POS: 0, 200, 400, 600, and 800 mg Zn-POS/kg diet. ZnSO4 was used to adjust to the desired amount of the Zn (80 mg/kg) in the Zn-POS diets. Broilers were fed with the experimental diets for 42 d including the starter (days 1 to 21) and grower (days 22 to 42) phases. Our results showed that dietary supplementation of Zn-POS linearly and quadratically increased (P < 0.05) the average daily gain and gain-to-feed ratio during 22 to 42 d and 1 to 42 d as well as body weight on day 42, whereas reduced (P < 0.05) the sum of mortality and lag abnormalities in broilers on day 42. Besides, serum alkaline phosphatase and copper-zinc superoxide dismutase activities increased (P < 0.05) linearly and quadratically in response to dietary Zn-POS supplemental level on day 42. Dietary Zn-POS supplementation increased Zn accumulation in serum (linear, P < 0.05), liver (linear, P < 0.05), and pancreas (linear and quadratic, P < 0.05). In addition, Zn-POS supplementation linearly and quadratically increased (P < 0.01, P < 0.05, respectively) MT concentrations in liver and pancreas of broilers. Pancreatic mRNA levels of MT, ZnT-1, and ZnT-2 increased (P < 0.05) linearly and quadratically, and the mRNA expression of metal response element-binding transcription factor-1 increased linearly (P < 0.05), in response to dietary Zn-POS supplementation. In conclusion, supplementation of Zn-POS in the diet increases Zn enrichment in the metabolic organs such as liver and pancreas and promotes productive performance in broilers.
本研究旨在探讨果胶锌寡糖螯合物（Zn-POS）对肉鸡生长性能、血清酶活性、组织锌积累、金属硫蛋白（MT）浓度和锌转运体（ZnT）基因表达的影响。将 540 只 1 日龄的爱拔益加肉鸡随机分配到 5 个日粮组，每个日粮组 6 个重复，每个重复 18 只鸡。日粮中添加相同的锌水平（80mg/kg 日粮），但 Zn-POS 的添加量不同：0、200、400、600 和 800mg Zn-POS/kg 日粮。用 ZnSO4 调整 Zn-POS 日粮中的 Zn 含量（80mg/kg）。肉鸡在 42 天的试验期中分别接受基础日粮（1-21 天）和生长日粮（22-42 天）的饲养。结果表明，日粮添加 Zn-POS 线性和二次增加（P＜0.05）了 22-42 天和 1-42 天的平均日增重和增重-饲料比，以及第 42 天的体重，同时降低了第 42 天肉鸡的死亡率和滞后异常率（P＜0.05）。此外，血清碱性磷酸酶和铜锌超氧化物歧化酶活性在第 42 天线性和二次增加（P＜0.05）响应于日粮 Zn-POS 的补充水平。日粮 Zn-POS 补充线性和二次增加（P＜0.05，P＜0.01）了血清（线性，P＜0.05）、肝脏（线性，P＜0.05）和胰腺（线性和二次，P＜0.05）中的锌积累。胰腺 MT、ZnT-1 和 ZnT-2 的 mRNA 水平线性和二次增加（P＜0.05），金属反应元件结合转录因子-1 的 mRNA 表达线性增加（P＜0.05），这与日粮 Zn-POS 的补充有关。综上所述，日粮中添加 Zn-POS 可增加肝脏和胰腺等代谢器官的锌富集，并促进肉鸡的生产性能。
