Shenep J L, Munson R S, Barenkamp S J, Granoff D M
Infect Immun. 1983 Oct;42(1):257-63. doi: 10.1128/iai.42.1.257-263.1983.
Serum antibody against polyribosylribitol phosphate, the capsular antigen of Haemophilus influenzae type b, confers protection against experimental Haemophilus infection. Antibodies against noncapsular antigens are also protective, but the antigenic specificity of the protective antibodies remains unknown. Antilipopolysaccharide antibody was prepared by immunization of rabbits with boiled H. influenzae type b cells. Antilipopolysaccharide antibodies present in these sera did not protect against experimental Haemophilus bacteremia in infant rats. Antisera were also prepared by immunization of rabbits with live H. influenzae type b bacteria. After absorption of anticapsular and antilipopolysaccharide antibodies, these sera contained antibody to several outer membrane proteins which were accessible on the intact bacterial surface as detected by radioimmune precipitation. These absorbed sera prevented experimental Haemophilus bacteremia in infant rats. Thus, antibodies against noncapsular, non-lipopolysaccharide determinants, possibly against one or more outer membrane proteins, confer protection against experimental H. influenzae type b disease. In contrast, antibodies against lipopolysaccharide are ineffective.
抗多聚核糖醇磷酸（b型流感嗜血杆菌的荚膜抗原）的血清抗体可抵御实验性流感嗜血杆菌感染。针对非荚膜抗原的抗体也具有保护作用，但保护性抗体的抗原特异性仍不清楚。通过用煮沸的b型流感嗜血杆菌细胞免疫兔子制备抗脂多糖抗体。这些血清中存在的抗脂多糖抗体不能保护幼鼠免受实验性流感嗜血杆菌菌血症的侵害。还通过用活的b型流感嗜血杆菌免疫兔子制备抗血清。在吸收抗荚膜和抗脂多糖抗体后，这些血清含有针对几种外膜蛋白的抗体，通过放射免疫沉淀法检测发现这些外膜蛋白在完整细菌表面是可及的。这些吸收后的血清可预防幼鼠的实验性流感嗜血杆菌菌血症。因此，针对非荚膜、非脂多糖决定簇，可能针对一种或多种外膜蛋白的抗体可抵御实验性b型流感嗜血杆菌疾病。相比之下，抗脂多糖抗体无效。
