Cardin L, Onesto J P, Moury B
INRA, URIH Phytopathologie, BP167, F-06903 Sophia-Antipolis cedex, France.
INRA, UR407 Pathologie Végétale, Domaine St Maurice, F-84143 Montfavet cedex, France.
Plant Dis. 2010 Jun;94(6):790. doi: 10.1094/PDIS-94-6-0790C.
Chinese peony (Paeonia lactiflora Pall.), a hardy ornamental plant of the family Paeoniaceae cultivated in gardens and for cut flower production, is frequently infected by Tobacco rattle virus (TRV) in the field. The virus usually induces severe mosaic and chlorotic ringspot symptoms in the leaves, decreasing the commercial value of cut flowers. TRV is routinely detected by mechanical inoculation onto Nicotiana tabacum cv Xanthi, where it induces typical necrotic local ringspots in 3 to 7 days, followed by a reverse transcription (RT)-PCR test (2). In 2004, Xanthi test plants inoculated with sap extracts from 4 of 36 P. lactiflora cv. Odile plants grown in a field plot in the region of Hyères (southeast France) showed systemic mosaic symptoms in addition to the TRV-typical response. In each case, Cucumber mosaic virus (CMV) was detected by the reactions of a range of inoculated plants (1), the observation of 30 nm isometric particles in crude leaf extracts with the electron microscope, and by positive reactions in double antibody sandwich (DAS)-ELISAs with specific polyclonal antibodies. In double-immunodiffusion analysis, these isolates were shown to belong to the group II of CMV isolates (3). ELISA of the peony plants confirmed the presence of CMV and revealed two additional infected plants in the spring of 2006. Following isolation from local lesions on Vigna unguiculata and multiplication in Xanthi tobacco plants, one of the isolates was used to inoculate manually or with Myzus persicae aphids 10 CMV-free plants of P. lactiflora cv. Odile obtained from meristem culture. Three months postinoculation, only three of the aphid-inoculated plants were CMV positive by DAS-ELISA. No change was observed at 1 year postinoculation and no symptoms have been observed, even in CMV-infected plants. CMV appears to be latent in P. lactiflora, therefore detection of CMV before vegetative propagation of the plants is advised because of the risks of synergism for symptoms with other viruses such as TRV. To our knowledge this is the first report of CMV in peony. References: (1) L. Cardin et al. Plant Dis. 87:1263, 2003. (2) D. J. Robinson J. Virol. Methods 40:55, 1992. (3) M. J. Roossinck. J. Virol. 76:3382, 2002.
芍药（Paeonia lactiflora Pall.）是芍药科一种耐寒的观赏植物，用于园林种植和切花生产，在田间常受烟草脆裂病毒（TRV）感染。该病毒通常在叶片上引发严重的花叶病和褪绿环斑症状，降低切花的商业价值。常规通过机械接种到烟草品种Xanthi上检测TRV，接种后3至7天它会在该品种上引发典型的坏死局部环斑，随后进行逆转录（RT）-PCR检测（2）。2004年，用来自法国东南部耶尔地区一块田间种植的36株芍药品种Odile中的4株的汁液提取物接种Xanthi检测植株，这些植株除出现TRV典型反应外，还表现出系统性花叶症状。在每种情况下，通过一系列接种植株的反应（1）、用电子显微镜在粗叶提取物中观察到30纳米等轴颗粒以及用特异性多克隆抗体进行双抗体夹心（DAS）-ELISA检测呈阳性反应，检测到黄瓜花叶病毒（CMV）。在双向免疫扩散分析中，这些分离物显示属于CMV分离物的II组（3）。对芍药植株的ELISA检测证实存在CMV，并在2006年春季发现另外两株受感染植株。从豇豆的局部病斑分离并在Xanthi烟草植株中繁殖后，其中一个分离物用于人工接种或用桃蚜接种10株从分生组织培养获得的无CMV的芍药品种Odile植株。接种三个月后，通过DAS-ELISA检测，仅3株经蚜虫接种的植株CMV呈阳性。接种1年后未观察到变化，即使在受CMV感染的植株中也未观察到症状。CMV似乎在芍药中呈潜伏状态，因此建议在植株营养繁殖前检测CMV，因为存在与其他病毒如TRV协同产生症状的风险。据我们所知，这是芍药中CMV的首次报道。参考文献：（1）L. Cardin等人，《植物病害》87:1263，2003年。（2）D. J. Robinson，《病毒学方法杂志》40:55，1992年。（3）M. J. Roossinck，《病毒学杂志》76:3382，2002年。
