Cardin L, Moury B
INRA, URIH Phytopathologie, BP167, F-06903 Sophia-Antipolis cedex, France.
INRA, Station de Pathologie Végétale, Domaine St Maurice, BP94, F-84143 Montfavet cedex, France.
Plant Dis. 2007 Nov;91(11):1516. doi: 10.1094/PDIS-91-11-1516C.
Echium candicans (Linn.) Herb. Banks (Pride of Madeira or Viper's Bugloss), family Boraginaceae, is a perennial shrub used in gardens for the ornamental quality of its deep blue inflorescences, especially in coastal areas near the Mediterranean Sea. Mosaic symptoms were observed in leaves of E. candicans in the Alpes Maritimes Department of southeastern France, St Jean Cap Ferrat in 1994, Menton in 2002, and Antibes in 2005. Symptoms exhibited in a range of inoculated plants including Nicotiana tabacum cvs. Xanthi and Samsun, Chenopodium quinoa, C. amaranticolor, Vigna unguiculata cv. Black, and Cucumis sativus cv. Poinsett were typical of Cucumber mosaic virus (CMV). Occurrence of CMV in one sample from each of the three localities was confirmed by the observation of isometric particles (approximately 30 nm) with the electron microscope in crude sap preparations from the infected plants, positive reactions in double-antibody sandwich (DAS)-ELISA to polyclonal antibodies raised against CMV (1), and the nonpersistent transmission of the virus from infected Xanthi to virus-free Xanthi plants by Myzus persicae. In double-immunodiffusion analysis, the three isolates were shown to belong to the CMV subgroup II (1,2). To determine if CMV was responsible for the symptoms observed, the isolate from Antibes was multiplied in Xanthi plants after isolation from local lesions on V. unguiculata and mechanically inoculated to 3-year-old plants of E. candicans tested to be free from CMV before the mechanical inoculation. One month after inoculation, mild mosaic symptoms were observed in young leaves and CMV was detected by DAS-ELISA in 10 of 10 inoculated plants. To our knowledge, this is the first report of CMV in E. candicans. References: (1) J.-C. Devergne and L. Cardin. Ann. Phytopathol. 7:225, 1975. (2) M. J. Roossinck. J. Virol. 76:3382, 2002.
深蓝蓟（Echium candicans (Linn.) Herb. Banks）（马德拉之傲或蝰蛇紫草），紫草科植物，是一种多年生灌木，因其深蓝色花序具有观赏价值，常用于园林造景，在地中海沿岸地区尤为常见。1994年在法国东南部滨海阿尔卑斯省的圣让卡普费拉、2002年在芒通以及2005年在昂蒂布，深蓝蓟的叶片上均观察到了花叶症状。在一系列接种植物上表现出的症状，包括烟草品种Xanthi和Samsun、藜麦、千日红、豇豆品种Black以及黄瓜品种Poinsett，均为黄瓜花叶病毒（CMV）的典型症状。通过电子显微镜观察感染植株粗汁液制备物中的等轴颗粒（约30纳米）、双抗体夹心（DAS）-ELISA对针对CMV产生的多克隆抗体呈阳性反应（1）以及桃蚜将病毒从感染的Xanthi非持久性传播至无病毒的Xanthi植株，证实了来自这三个地点的每个地点的一个样本中均存在CMV。在双向免疫扩散分析中，这三个分离株显示属于CMV亚组II（1,2）。为确定CMV是否是观察到的症状的病因，将来自昂蒂布的分离株从豇豆上的局部病斑分离后在Xanthi植株上扩繁，并机械接种到3年生的深蓝蓟植株上，这些植株在机械接种前经检测无CMV。接种一个月后，在幼叶上观察到轻度花叶症状，并且通过DAS-ELISA在10株接种植株中的10株中检测到了CMV。据我们所知，这是关于CMV在深蓝蓟中的首次报道。参考文献：（1）J.-C. Devergne和L. Cardin。《植物病理学纪事》7:225，1975年。（2）M. J. Roossinck。《病毒学杂志》76:3382，2002年。
