Durham T C, Baker C, Jones L, Snyder L Unruh
Department of Biological Sciences, Agriculture Program, Nicholls State University, Thibodaux, LA 70310.
Florida Division of Plant Industry, 1911 SW 34th St., Gainesville 32608.
Plant Dis. 2010 Apr;94(4):487. doi: 10.1094/PDIS-94-4-0487B.
In October 2006, snap bean (Phaseolus vulgaris 'Titan') plants in an Alachua County field exhibited symptoms of foliar mottling, puckering, and curl. Symptomatic plants were distributed along field margins infested with whiteflies (100% incidence). Six collected leaf specimens all tested positive for nuclear inclusion bodies typical of begomoviruses with the methodology outlined by Christie et al. (1). To confirm the putative begomovirus association, total DNA was extracted with Qiagen's DNeasy Plant Mini Kit (Qiagen, Valencia, CA). The degenerate Begomovirus primers 5'-GCCCACATYGTCTTYCCNGT-3' and 5'-GGCTTYCTRTACATRGG-3' were used to amplify a 1.1-kb fragment of DNA-A (2). Primers SiGMVf 5'-CCTAAGCGCGATTTGCCAT-3' and SiGMVr 5'-TACAGGGAGCTAAATCCAGCT-3' were designed to amplify the remaining 1.5 kb of the DNA-A component. The sequence of both PCR products was compiled to generate a complete sequence of an A component (2,633 nt). BLAST analysis of this sequence (GenBank Accession No. GQ357649) isolated from snap bean indicated 95% nucleotide identity to Sida golden mosaic virus (SiGMV) (GenBank Accession No. AF049336) isolated from Sida santaremensis from Florida. To our knowledge, this is the first report of SiGMV in Florida snap beans. Further study is warranted to examine the etiological and economic implications of this finding. References: (1) R. G. Christie et al. Phytopathology 76:124, 1986. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993.
2006年10月，阿拉楚阿县一块田地中的食荚菜豆（菜豆品种‘泰坦’）植株出现了叶片斑驳、起皱和卷曲的症状。有症状的植株分布在受粉虱侵害的田边（发病率100%）。按照克里斯蒂等人（1）概述的方法，采集的6份叶片标本对典型双生病毒核内含体的检测均呈阳性。为了确认假定的双生病毒关联，使用Qiagen公司的DNeasy植物微量提取试剂盒（Qiagen，加利福尼亚州瓦伦西亚）提取总DNA。使用简并双生病毒引物5'-GCCCACATYGTCTTYCCNGT-3'和5'-GGCTTYCTRTACATRGG-3'扩增DNA-A的1.1 kb片段（2）。设计引物SiGMVf 5'-CCTAAGCGCGATTTGCCAT-3'和SiGMVr 5'-TACAGGGAGCTAAATCCAGCT-3'扩增DNA-A组分剩余的1.5 kb。将两个PCR产物的序列进行拼接，生成一个A组分的完整序列（2633 nt）。对从食荚菜豆中分离出的该序列（GenBank登录号GQ357649）进行BLAST分析表明，其与从佛罗里达州的圣塔伦 sida中分离出的 sida金色花叶病毒（SiGMV）（GenBank登录号AF049336）有95%的核苷酸同一性。据我们所知，这是佛罗里达州食荚菜豆中首次报道SiGMV。有必要进一步研究以探讨这一发现的病因学和经济影响。参考文献：（1）R.G.克里斯蒂等人，《植物病理学》76:124，1986年。（2）M.R.罗哈斯等人，《植物病害》77:340，1993年。
