Department of Entomology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA.
Department of Plant Pathology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA.
Viruses. 2023 Jan 26;15(2):357. doi: 10.3390/v15020357.
Sida golden mosaic virus (SiGMV) was first detected from snap bean ( L.) in Florida in 2006 and recently in Georgia in 2018. Since 2018, it has caused significant economic losses to snap bean growers in Georgia. This study, using a SiGMV isolate field-collected from prickly sida ( L.), examined the putative host range, vector-mediated transmission, and SiGMV-modulated effects on host-vector interactions. In addition, this study analyzed the phylogenetic relationships of SiGMV with other begomoviruses reported from spp. Host range studies confirmed that SiGMV can infect seasonal crops and perennial weed species such as snap bean, hollyhock ( L.) marsh mallow L.), okra ( (L.) Moench), country mallow ( L.), prickly sida (), and tobacco ( L.). The incidence of infection ranged from 70 to 100%. SiGMV-induced symptoms and virus accumulation varied between hosts. The vector, Gennadius, was able to complete its life cycle on all plant species, irrespective of SiGMV infection status. However, SiGMV infection in prickly sida and country mallow positively increased the fitness of whiteflies, whereas SiGMV infection in okra negatively influenced whitefly fitness. Whiteflies efficiently back-transmitted SiGMV from infected prickly sida, hollyhock, marsh mallow, and okra to snap bean, and the incidence of infection ranged from 27 to 80%. Complete DNA-A sequence from this study shared 97% identity with SiGMV sequences reported from Florida and it was determined to be closely related with sida viruses reported from the New World. These results suggest that SiGMV, a New World begomovirus, has a broad host range that would allow its establishment in the farmscapes/landscapes of the southeastern United States and is an emerging threat to snap bean and possibly other crops.
感染豆科作物的 Sida 金纹花叶病毒的鉴定与分析
Sida 金纹花叶病毒（SiGMV）于 2006 年首次从佛罗里达州的菜豆中被发现，最近又于 2018 年在佐治亚州被检测到。自 2018 年以来，它给佐治亚州的菜豆种植者造成了巨大的经济损失。本研究使用从刺叶山蚂蝗中采集的 SiGMV 分离株，检测了假定的宿主范围、媒介介导的传播以及 SiGMV 对宿主-媒介相互作用的调制效应。此外，本研究还分析了与其他报道的 属伴生病毒的 SiGMV 之间的系统发育关系。宿主范围研究证实，SiGMV 可感染季节性作物和多年生杂草物种，如菜豆、蜀葵（L.）、陆地锦葵（L.）、秋葵（（L.）Moench）、田菁（L.）、刺叶山蚂蝗（）和烟草（L.）。感染率为 70%至 100%。SiGMV 诱导的症状和病毒积累在不同宿主之间存在差异。烟粉虱（Bemisia tabaci）能够在所有植物物种上完成其生命周期，无论 SiGMV 感染状态如何。然而，SiGMV 感染刺叶山蚂蝗和田菁会增加粉虱的适合度，而 SiGMV 感染秋葵则会降低粉虱的适合度。粉虱能够有效地将 SiGMV 从受感染的刺叶山蚂蝗、蜀葵、陆地锦葵和秋葵中反向传播到菜豆上，感染率为 27%至 80%。本研究中的完整 DNA-A 序列与佛罗里达州报道的 SiGMV 序列具有 97%的同一性，它与从新世界报道的山蚂蝗病毒密切相关。这些结果表明，SiGMV 是一种新世界的伴生病毒，具有广泛的宿主范围，使其能够在美国东南部的农田/景观中建立，并对菜豆和可能其他作物构成新的威胁。
