Kumari S G, Attar N, Mustafayev E, Akparov Z
Virology Laboratory, International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria.
Azerbaijan National Academy Science of Genetic Resources Institute, 155 Azadliq Ave, 1106, Baku, Azerbaijan.
Plant Dis. 2009 Nov;93(11):1220. doi: 10.1094/PDIS-93-11-1220C.
A total of 482 chickpea (Cicer arietinum L.), 182 lentil (Lens culinaris Medik.), 12 vetch (Vicia sativa L.), 5 field pea (Pisum sativum L.), and 3 faba bean (Vicia faba L.) samples were collected from plants with symptoms suggestive of a viral infection (leaf rolling, yellowing, and stunting) from the major legume-production areas of Azerbaijan in the 2007 and 2008 growing seasons. All samples were tested by the tissue-blot immunoassay (3) at the Virology Laboratory of ICARDA, Syria using 11 specific legume virus antisera including a monoclonal antibody (2-5H9) (1) for Faba bean necrotic yellows virus (FBNYV). Laboratory tests showed that FBNYV was detected in 73, 61, 11, 3, and 2 samples of chickpea, lentil, vetch, field pea, and faba bean, respectively. Total DNA was extracted from six FBNYV-positive samples (two chickpea, two lentil, and two vetch) and tested by PCR with the following four primer sets (FBNYV, Milk vetch dwarf virus [MDV], Subterranean clover stunt virus [SCSV], and nanovirus DNA-R primers [F103 and R101]) (2). All six Azeri samples as well as the reference nanovirus isolates (SCSV-Australia, MDV-Japan, and FBNYV-Syria) generated amplicons of the expected size (~770 bp) using the nanovirus DNA-R primers (F103 & R101). In addition, Azeri samples and FBNYV-Syria yielded a PCR amplicon of the expected size (666 bp) with the FBNYV primer pair. The MDV- and SCSV-specific primers did not generate amplicons with these six samples. Sequence analysis of the FBNYV amplicons from two isolates (AzL 282-07 from lentil [GenBank Accession No. GQ351600] and AzV 277-07 from vetch [GenBank Accession No. GQ371215]) showed that they were 99% identical with each other. Comparing the sequence of AzL 282-07 with that of other nanoviruses revealed identities of 97% (FBNYV-Spain; DQ830990), 96% (FBNYV-Iran; AM493900), 92% (FBNYV-Syria; Y11408), 92% (FBNYV-Egypt; AJ132183), 78% (MDV; AB044387) and 69% (SCSV-Australia; U16734). FBNYV has been reported to infect food legumes in many countries in West Asia and North Africa and cause economic losses on faba bean in Egypt, Jordan, and Syria. To our knowledge, this is the first record of FBNYV infecting legume crops in Azerbaijan. References: (1) A. Franz et al. Ann. Appl. Biol. 128:255, 1996. (2) S. G. Kumari et al. Phytopathol. Mediterr. 47:42, 2008. (3) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994.
2007年和2008年生长季，从阿塞拜疆主要豆类产区有病毒感染症状（叶片卷曲、发黄和生长受阻）的植株上，共采集了482份鹰嘴豆（Cicer arietinum L.）、182份小扁豆（Lens culinaris Medik.）、12份巢菜（Vicia sativa L.）、5份豌豆（Pisum sativum L.）和3份蚕豆（Vicia faba L.）样本。所有样本均在叙利亚国际干旱地区农业研究中心病毒学实验室采用组织印迹免疫分析法(3)进行检测，使用了11种特异性豆类病毒抗血清，包括一种针对蚕豆坏死黄化病毒（FBNYV）的单克隆抗体（2-5H9）(1)。实验室检测表明，分别在73份鹰嘴豆、61份小扁豆、11份巢菜、3份豌豆和2份蚕豆样本中检测到FBNYV。从6份FBNYV阳性样本（2份鹰嘴豆、2份小扁豆和2份巢菜）中提取总DNA，并使用以下四组引物（FBNYV、紫云英矮缩病毒[MDV]、三叶草矮化病毒[SCSV]和纳米病毒DNA-R引物[F103和R101]）(2)进行PCR检测。所有6份阿塞拜疆样本以及参考纳米病毒分离株（SCSV-澳大利亚、MDV-日本和FBNYV-叙利亚）使用纳米病毒DNA-R引物（F103和R101）均产生了预期大小（约770 bp）的扩增子。此外，阿塞拜疆样本和FBNYV-叙利亚使用FBNYV引物对产生了预期大小（666 bp）的PCR扩增子。MDV和SCSV特异性引物对这6份样本未产生扩增子。对来自两个分离株（小扁豆的AzL 282-07 [GenBank登录号GQ351600]和巢菜的AzV 277-07 [GenBank登录号GQ371215]）的FBNYV扩增子进行序列分析表明，它们彼此间的同源性为99%。将AzL 282-07的序列与其他纳米病毒的序列进行比较，结果显示其与FBNYV-西班牙（DQ830990）的同源性为97%、与FBNYV-伊朗（AM493900）的同源性为96%、与FBNYV-叙利亚（Y11408）的同源性为92%、与FBNYV-埃及（AJ132183）的同源性为92%、与MDV（AB044387）的同源性为78%、与SCSV-澳大利亚（U16734）的同源性为69%。据报道，FBNYV在西亚和北非的许多国家感染食用豆类，并在埃及、约旦和叙利亚给蚕豆造成经济损失。据我们所知，这是FBNYV在阿塞拜疆感染豆类作物的首次记录。参考文献：(1) A. Franz等人，《应用生物学年鉴》128:255，1996年。(2) S. G. Kumari等人，《地中海植物病理学》47:42，2008年。(3) K. M. Makkouk和A. Comeau，《欧洲植物病理学杂志》100:71，1994年。
Zotero 插件