Tadesse N, Ali K, Gorfu D, Abraham A, Lencho A, Ayalew M, Yusuf A, Makkouk K M, Kumari S G
EARO, Addis Ababa, Ethiopia.
Alemaya University of Agriculture, Dire Dawa, Ethiopia.
Plant Dis. 1999 Jun;83(6):589. doi: 10.1094/PDIS.1999.83.6.589B.
A survey conducted during November 14-23, 1998, to identify viruses infecting chickpea (Cicer arietinum) and lentil (Lens culinaris) crops in the Shewa province of Ethiopia covered 33 chickpea and 32 lentil fields randomly selected. Identity of the viruses present and virus incidence were determined on the basis of laboratory testing of 100 to 200 randomly collected samples in addition to 15 to 20 symptomatic samples from each field. A total of 5,427 lentil and 3,836 chickpea samples were collected and tested for the presence of 12 different viruses by tissue blot immunoassay (1) at the Plant Pathology Laboratory in Debre Zeit Agriculture Research Center, Ethiopia. All antisera were virus specific, including those for beet western yellows virus (BWYV; ATCC PVAS-647) and soybean dwarf virus (SbDV; ATCC PVAS-650). More than 21% of the samples from 5 chickpea fields were infected; the most common virus was BWYV. Also, at least 21% of the samples from 11 lentil fields were virus positive; the most widespread virus was PSbMV. Highest rates of infection: of lentil in a single field, PSbMV in 58.5% of the samples; in a chickpea field, 41.3% of the samples positive for BWYV. Other viruses such as faba bean necrotic yellows nanovirus (FBNYV) and broad bean wilt fabavirus in chickpea and FBNYV, broad bean stain comovirus, bean yellow mosaic potyvirus, and cucumber mosaic cucumovirus in lentil were detected at very low incidence. Reference: (1) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994.
1998年11月14日至23日期间，在埃塞俄比亚的绍阿省开展了一项调查，以确定感染鹰嘴豆（Cicer arietinum）和小扁豆（Lens culinaris）作物的病毒。该调查随机选取了33块鹰嘴豆田和32块小扁豆田。除了从每块田地采集15至20个有症状的样本外，还对随机采集的100至200个样本进行实验室检测，以此确定存在的病毒种类及病毒发生率。在埃塞俄比亚德布雷泽特农业研究中心的植物病理实验室，共采集了5427个小扁豆样本和3836个鹰嘴豆样本，并通过组织印迹免疫分析（1）检测是否存在12种不同的病毒。所有抗血清均针对特定病毒，包括针对甜菜西方黄化病毒（BWYV；ATCC PVAS - 647）和大豆矮缩病毒（SbDV；ATCC PVAS - 650）的抗血清。来自5块鹰嘴豆田的样本中，超过21%受到感染；最常见的病毒是BWYV。此外，来自11块小扁豆田的样本中，至少21%呈病毒阳性；分布最广的病毒是豌豆耳突花叶病毒（PSbMV）。感染率最高的情况：在单块小扁豆田中，PSbMV在58.5%的样本中出现；在一块鹰嘴豆田中，41.3%的样本对BWYV呈阳性。还检测到其他病毒，如鹰嘴豆中的蚕豆坏死黄化纳米病毒（FBNYV）和蚕豆萎蔫蚕豆病毒，以及小扁豆中的FBNYV、蚕豆斑驳豇豆花叶病毒、菜豆黄花叶马铃薯Y病毒和黄瓜花叶黄瓜病毒，但其发生率极低。参考文献：（1）K. M. Makkouk和A. Comeau。《欧洲植物病理学杂志》100:71，1994年。
