DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, 15121 Alessandria, Italy.
Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy.
Int J Mol Sci. 2019 Feb 11;20(3):764. doi: 10.3390/ijms20030764.
Since Biblical times, honey has been utilized in "folk medicine", and in recent decades the positive qualities of honey have been re-discovered and are gaining acceptance. Scientific literature states that honey has been successfully utilized on infections not responding to classic antiseptic and antibiotic therapy, because of its intrinsic H₂O₂ production. In our study, we demonstrated the involvement of H₂O₂ as a main mediator of honey regenerative effects on an immortalized human keratinocyte cell line. We observed that this extracellularly released H₂O₂ could pass across the plasma membrane through a specific aquaporin (i.e., AQP3). Once in the cytoplasm H₂O₂, in turn, induces the entry of extracellular Ca through Melastatin Transient Receptor Potential 2 (TRPM2) and Orai1 channels. Honey-induced extracellular Ca entry results in wound healing, which is consistent with the role played by Ca signaling in tissue regeneration. This is the first report showing that honey exposure increases intracellular Ca concentration ([Ca]), due to H₂O₂ production and redox regulation of Ca-permeable ion channels, opening up a new horizon for the utilization of the honey as a beneficial tool.
自古以来，蜂蜜就被应用于“民间医学”，近几十年来，蜂蜜的积极特性被重新发现并逐渐被人们所接受。科学文献表明，由于蜂蜜自身产生的 H₂O₂，它在对抗生素和传统防腐剂治疗无效的感染方面已被成功应用。在我们的研究中，我们证明 H₂O₂作为一种主要介质，参与了蜂蜜对永生化人角质形成细胞系的再生作用。我们观察到，这种细胞外释放的 H₂O₂可以通过特定的水通道蛋白（即 AQP3）穿过质膜。进入细胞质后，H₂O₂反过来通过 Melastatin 瞬时受体电位 2（TRPM2）和 Orai1 通道诱导细胞外 Ca 的进入。蜂蜜诱导的细胞外 Ca 进入导致伤口愈合，这与 Ca 信号在组织再生中的作用一致。这是第一项表明蜂蜜暴露会由于 H₂O₂的产生和 Ca 通透性离子通道的氧化还原调节而增加细胞内 Ca 浓度([Ca])的报告，为将蜂蜜作为有益工具的应用开辟了新的前景。
