Jontell M, Gäbel H, Ohman S C, Brynger H
Department of Oral Diagnosis, Faculty of Odontology, University of Gothenburg, Sweden.
Transpl Int. 1988 Dec;1(4):186-9. doi: 10.1111/j.1432-2277.1988.tb01813.x.
This study was designed to investigate the influence of systemic immunosuppressive therapy on the HLA-DR expression of epidermal Langerhans cells. Fifteen renal allograft recipients immunosuppressed with cyclosporin A and steroids were studied. Skin biopsies were taken from the upper arm prior to transplantation and at different intervals during the post-transplantation period. The epidermis was separated from the dermis, and the epidermal sheet was subjected to immunohistochemistry in order to make the HLA-DR antigens on the Langerhans cells visible. Following 2 days of immunosuppression, the number of Langerhans cells expressing HLA-DR antigens started to decrease and after 1 week, only 60% of the initial number of positive cells were detected. The number of positive cells remained low throughout the experimental period. It is suggested that systemic immunosuppressive therapy will suppress the expression of HLA-DR antigens on epidermal Langerhans cells, something which may mirror a systemic effect on other antigen-presenting cells.
本研究旨在探讨全身免疫抑制治疗对表皮朗格汉斯细胞HLA - DR表达的影响。研究对象为15例接受环孢素A和类固醇免疫抑制治疗的肾移植受者。在移植前及移植后的不同时间段，从患者上臂取皮肤活检样本。将表皮与真皮分离，对表皮片进行免疫组织化学检测，以使朗格汉斯细胞上的HLA - DR抗原可见。免疫抑制治疗2天后，表达HLA - DR抗原的朗格汉斯细胞数量开始减少，1周后，仅检测到初始阳性细胞数量的60%。在整个实验期间，阳性细胞数量一直维持在较低水平。提示全身免疫抑制治疗会抑制表皮朗格汉斯细胞上HLA - DR抗原的表达，这可能反映出对其他抗原呈递细胞的全身效应。
