Marks T R, Simpson S E
Crop Science Department, Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, UK e-mail:
Plant Cell Rep. 2000 Dec;19(12):1171-1176. doi: 10.1007/s002990000264.
Differences in rhizogenesis between easy-to-root Forsythia×intermedia Zab. cv. Lynwood and difficult-to-root Syringa vulgaris L. cv. Madame Lemoine were measured in an experimental system based upon internodal stem sections excised from axillary shoot cultures. Root induction in Syringa was distinctly polar, responding best to distal application of indole-3-butyric acid (IBA), whereas Forsythia was equally responsive to IBA applied at either end. Root initiation in Syringa declined rapidly from 73% to 32% over 48 h when the application of a 24-h pulse of IBA was delayed following excision of the internode; in Forsythia a smaller decline (93-70%) occurred over 144 h. Forsythia internodes were the more responsive, and developed roots after distal or proximal application of 3 μM IBA, whereas Syringa required the distal application of 30 μM IBA.
在一个基于从腋芽培养物中切取的节间茎段的实验系统中，对易于生根的金钟连翘（Forsythia×intermedia Zab. cv. Lynwood）和难以生根的丁香（Syringa vulgaris L. cv. Madame Lemoine）的生根差异进行了测定。丁香的生根明显具有极性，对吲哚 - 3 - 丁酸（IBA）在远端施用反应最佳，而金钟连翘对在两端施用IBA的反应相同。当节间切除后延迟24小时脉冲施用IBA时，丁香的生根起始在48小时内从73%迅速下降到32%；在金钟连翘中，在144小时内出现较小的下降（从93%降至70%）。金钟连翘节间反应更灵敏，在远端或近端施用3 μM IBA后即可生根，而丁香需要远端施用30 μM IBA才能生根。
