Buffalo Neuroimaging Analysis Center, Department of Neurology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA.
Department of Pharmaceutical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA.
Mult Scler. 2020 Mar;26(3):322-332. doi: 10.1177/1352458519828667. Epub 2019 Feb 13.
Epstein-Barr virus (EBV) infection has been associated with higher clinical activity and risk of multiple sclerosis (MS).
To evaluate associations between EBV-specific humoral response and magnetization transfer ratio (MTR)-derived measure in MS patients and healthy controls (HCs).
The study included 101 MS patients (69 relapsing-remitting multiple sclerosis (RRMS) and 32 secondary-progressive multiple sclerosis (SPMS)) and 41 HCs who underwent clinical, serological, and magnetic resonance imaging (MRI) investigations. MTR values of T1 or T2 lesion volume (LV), normal-appearing (NA) brain tissue (NABT), gray matter (NAGM), and white matter (NAWM) were obtained. Enzyme-linked immunosorbent assay was used to quantify EBV antibody levels. Partial correlations corrected for MRI strength were used, and Benjamini-Hochberg-adjusted -values < 0.05 were considered significant.
MS patients had significantly higher anti-EBV nuclear antigen-1 (EBNA-1) titer when compared to HCs (107.9 U/mL vs 27.8 U/mL, < 0.001). Within the MS group, higher serum anti-EBNA-1 titer was significantly correlated with lower T1-LV MTR ( = -0.287, = 0.035). Within the RRMS group, higher serum anti-EBNA-1 titer was associated with T1-LV MTR ( = -0.524, = 0.001) and NAGM MTR ( = -0.308, = 0.043). These associations were not present in HCs or SPMS patients.
Greater EBV humoral response is associated with lower GM MTR changes and focal destructive lesion pathology in RRMS patients.
EB 病毒（EBV）感染与多发性硬化症（MS）的更高临床活性和风险有关。
评估 EBV 特异性体液反应与 MS 患者和健康对照者（HC）磁化传递率（MTR）衍生测量值之间的相关性。
本研究纳入了 101 名 MS 患者（69 名复发缓解型多发性硬化症（RRMS）和 32 名继发进展型多发性硬化症（SPMS））和 41 名 HC，进行了临床、血清学和磁共振成像（MRI）检查。获得 T1 或 T2 病变体积（LV）、正常外观（NA）脑实质（NABT）、灰质（NAGM）和白质（NAWM）的 MTR 值。采用酶联免疫吸附试验（ELISA）定量 EBV 抗体水平。使用校正 MRI 强度的偏相关分析，校正后的 P 值<0.05 认为具有统计学意义。
与 HC 相比，MS 患者的 EBV 核抗原-1（EBNA-1）滴度明显更高（107.9 U/mL 比 27.8 U/mL，P<0.001）。在 MS 组中，血清抗-EBNA-1 滴度越高，T1-LV MTR 越低（r=-0.287，P=0.035）。在 RRMS 组中，血清抗-EBNA-1 滴度与 T1-LV MTR（r=-0.524，P=0.001）和 NAGM MTR（r=-0.308，P=0.043）呈负相关。这些相关性在 HC 或 SPMS 患者中不存在。
RRMS 患者 EBV 体液反应增强与 GM MTR 变化和局灶性破坏性病变病理学有关。
