Abutaha Nael, Nasr Fahd A, Mohammed Al-Zahrani, Semlali AbdelHabib, Al-Mekhlafi Fahd A, Wadaan Mohamed A
Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Medicinal Aromatic, and Poisonous Plants Research Centre, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
Mol Biol Rep. 2019 Apr;46(2):2187-2196. doi: 10.1007/s11033-019-04672-3. Epub 2019 Feb 11.
Calendula arvensis L. is used in traditional folk medicine for the treatment of several diseases. Leaves, stems, and flowers of C. arvensis were extracted using a Soxhlet extractor with different solvents (i.e., hexane, chloroform, ethyl acetate, and methanol). The ethyl acetate extract of C. arvensis flowers (CAF EtOAC) had cytotoxic activity against MCF-7 and MDA-MB-231 breast cancer cells, with IC values of 70 and 78 µg/mL, respectively. Microscopic examination revealed concentration-dependent cell shrinkage, cell detachment, nuclear fragmentation, and chromatin condensation. The CAF EtOAC inhibited the migration of cultured cells in a scratch wounding assay, indicating a possible defense against metastasis. The same extract also caused apoptosis by downregulating Bcl-2 and upregulating Bax and caspase 3/7 activity. Phytochemical analyses revealed the presence of phenols and flavonoids, and gas chromatography-mass spectroscopy (GC-MS) revealed a high content of linolenic acid in the extract. Based on our data, the CAF EtOAC may provide active ingredients for the development of novel chemotherapeutics for breast cancer therapy.
田野金盏花（Calendula arvensis L.）在传统民间医学中用于治疗多种疾病。使用索氏提取器用不同溶剂（即己烷、氯仿、乙酸乙酯和甲醇）提取田野金盏花的叶、茎和花。田野金盏花花的乙酸乙酯提取物（CAF EtOAC）对MCF-7和MDA-MB-231乳腺癌细胞具有细胞毒性活性，IC值分别为70和78 µg/mL。显微镜检查显示出浓度依赖性的细胞收缩、细胞脱离、核碎裂和染色质浓缩。在划痕损伤试验中，CAF EtOAC抑制培养细胞的迁移，表明其可能具有抗转移作用。同一提取物还通过下调Bcl-2和上调Bax以及caspase 3/7活性诱导细胞凋亡。植物化学分析表明存在酚类和黄酮类化合物，气相色谱-质谱联用（GC-MS）显示提取物中含有高含量的亚麻酸。基于我们的数据，CAF EtOAC可能为开发用于乳腺癌治疗的新型化学治疗药物提供活性成分。
