Abudunia A-M, Marmouzi I, Faouzi M E A, Ramli Y, Taoufik J, El Madani N, Essassi E M, Salama A, Khedid K, Ansar M, Ibrahimi A
Department of Biotechnology Laboratory (Med-Biotech), Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco; Department of Medicinal Chemistry Laboratory, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.
Laboratoire de pharmacologie et toxicologie, équipe de pharmacocinétique, University Mohammed V in Rabat, faculté de médicine et pharmacie, Rabat Instituts, BP 6203, Rabat, Morocco.
J Mycol Med. 2017 Mar;27(1):90-97. doi: 10.1016/j.mycmed.2016.11.002. Epub 2016 Dec 20.
Calendula arvensis (CA) is one of the important plants used in traditional medicine in Morocco, due to its interesting chemical composition. The present study aimed to determine the anticandidal, antioxidant and antibacterial activities, and the effects of extracts of CA flowers on the growth of myeloid cancer cells. Also, to characterize the chemical composition of the plant. Flowers of CA were collected based on ethnopharmacological information from the villages around the region Rabat-Khemisset, Moroccco. The hexane and methanol extracts were obtained by soxhlet extraction, while aqueous extracts was obtained by maceration in cold water. CA extracts were assessed for antioxidant activity using four different methods (DPPH, FRAP, TEAC, β-carotene bleaching test). Furthermore, the phenolic and flavonoid contents were measured, also the antimicrobial activity has been evaluated by the well diffusion method using several bacterial and fungal strains. Finally, extracts cytotoxicity was assessed using MTT test. Phytochemical quantification of the methanolic and aqueous extracts revealed that they were rich with flavonoid and phenolic content and were found to possess considerable antioxidant activities. MIC values of methanolic extracts were 12.5-25μg/mL. While MIC values of hexanolic extracts were between 6.25-12.5μg/mL and were bacteriostatic for all bacteria while methanolic and aqueous extracts were bactericidal. In addition, the extracts exhibited no activity on Candida species except the methanolic extract, which showed antifungal activity onCandida tropicalis 1 and Candida famata 1. The methanolic and aqueous extracts also exhibited antimyeloid cancer activity (IC of 31μg/mL). In our study, we conclude that the methanolic and aqueous extracts were a promising source of antioxidant, antimicrobial and cytotoxic agents.
田野金盏花（CA）是摩洛哥传统医学中使用的重要植物之一，因其化学成分有趣。本研究旨在确定其抗念珠菌、抗氧化和抗菌活性，以及CA花提取物对髓系癌细胞生长的影响。此外，还对该植物的化学成分进行表征。根据民族药理学信息，从摩洛哥拉巴特-海米萨特地区周边村庄采集了CA花。通过索氏提取法获得己烷和甲醇提取物，而水提取物通过冷水浸渍法获得。使用四种不同方法（DPPH、FRAP、TEAC、β-胡萝卜素漂白试验）评估CA提取物的抗氧化活性。此外，还测定了酚类和黄酮类含量，并且使用几种细菌和真菌菌株通过打孔扩散法评估了抗菌活性。最后，使用MTT试验评估提取物的细胞毒性。甲醇提取物和水提取物的植物化学定量分析表明，它们富含黄酮类和酚类成分，并具有相当的抗氧化活性。甲醇提取物的最低抑菌浓度（MIC）值为12.5 - 25μg/mL。而己醇提取物的MIC值在6.25 - 12.5μg/mL之间，对所有细菌均有抑菌作用，而甲醇提取物和水提取物具有杀菌作用。此外，除甲醇提取物对热带假丝酵母1和法马假丝酵母1显示出抗真菌活性外，提取物对念珠菌属均无活性。甲醇提取物和水提取物还表现出抗髓系癌活性（半数抑制浓度为31μg/mL）。在我们的研究中，我们得出结论，甲醇提取物和水提取物是抗氧化剂、抗菌剂和细胞毒性剂的有前景来源。
