Chen Ling-Ling, Hu Ting, Xing Zi-Yu, Lu Jia-Sen, Yang Han, Huang Xing
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
Int J Syst Evol Microbiol. 2019 Apr;69(4):1130-1134. doi: 10.1099/ijsem.0.003281. Epub 2019 Feb 13.
A yellow-coloured bacterial strain, designated F-4, was isolated from a farmland soil sample from Qianshan, Anhui province, China. Strain F-4 was Gram-stain-negative, strictly aerobic, oval-shaped, motile (by gliding) and non-spore-forming. Growth occurred at 20-35 °C (optimum, 30 °C), at pH 6.0-8.0 (pH 7.0) and with 0-1.0 % (w/v) NaCl (0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain F-4 belonged to the genus Taibaiella. 16S rRNA gene sequence similarity values between strain F-4 and the type strains of the three recognized species of the genus Taibaiella, Taibaiella koreensis KACC 17171, Taibaiella soli KCTC 42277 and Taibaiella chishuiensis JCM 19637, were 98.1, 96.4 and 95.9 %, respectively. The predominant respiratory quinone was MK-7, with MK-8 as a minor component. The major polar lipids of strain F-4 were three unidentified lipids, two unidentified aminolipids, three unidentified phospholipids, an unidentified aminophospholipid and phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0. The G+C content of the genomic DNA based on total genome calculations was 51.3 mol%. The major polyamine of strain F-4 was homospermidine. The average nucleotide identity and the digital DNA-DNA hybridization values for draft genomes between strain F-4 and strain THG-DT86 were 79.8 and 22.6 %, respectively. On the basis of the genotypic and phenotypic data presented here, strain F-4 represents a novel species of the genus Taibaiella, for which the name Taibaiella helva sp. nov. is proposed. The type strain is F-4 (=KCTC 62442=CGMCC 1.13562).
从中国安徽省潜山市的农田土壤样本中分离出一株黄色的细菌菌株，命名为F-4。菌株F-4革兰氏染色阴性，严格需氧，椭圆形，具运动性（通过滑动），不形成芽孢。在20-35 °C（最适温度为30 °C）、pH 6.0-8.0（pH 7.0）和0-1.0 %（w/v）NaCl（0 %）条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株F-4属于太白杆菌属。菌株F-4与太白杆菌属三个已确认物种的模式菌株，即韩国太白杆菌KACC 17171、土壤太白杆菌KCTC 42277和赤水太白杆菌JCM 19637之间的16S rRNA基因序列相似性值分别为98.1%、96.4%和95.9%。主要呼吸醌为MK-7，MK-8为次要成分。菌株F-4的主要极性脂类是三种未鉴定的脂类、两种未鉴定的氨基脂类、三种未鉴定的磷脂、一种未鉴定的氨基磷脂和磷脂酰乙醇胺。主要细胞脂肪酸为异-C15 : 0、异-C15 : 1 G和异-C17 : 0。基于全基因组计算的基因组DNA的G+C含量为51.3 mol%。菌株F-4的主要多胺是高亚精胺。菌株F-4与菌株THG-DT86的草图基因组之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为79.8%和22.6%。基于本文提供的基因型和表型数据，菌株F-4代表太白杆菌属的一个新物种，为此提出名为太白淡黄杆菌（Taibaiella helva sp. nov.）。模式菌株为F-4（=KCTC 62442=CGMCC 1.13562）。
