采用分光光度酶免疫分析法测定干血斑中的茶碱。
Measurement of theophylline in dried blood spots by spectrophotometric enzyme immunoassay.
作者信息
Rattenbury J M, Taylor T
机构信息
Department of Chemical Pathology, Children's Hospital, Western Bank, Sheffield, UK.
出版信息
Ann Clin Biochem. 1988 Nov;25 ( Pt 6):650-3. doi: 10.1177/000456328802500609.
A widely-used enzyme immunoassay for the measurement of theophylline in plasma (EMIT) has been adapted for use with dried blood spots. Potential interference from haemoglobin in eluates of the spots was avoided by autoclaving them prior to analysis. The method was investigated in terms of the recovery of theophylline added to samples, precision, and the correlation of results in DBS with those in plasma samples. The recoveries of some other drugs from eluates of autoclaved DBS were also investigated.
一种广泛用于测量血浆中茶碱的酶免疫测定法（EMIT）已被改编用于干血斑检测。通过在分析前对血斑洗脱液进行高压灭菌，避免了血红蛋白的潜在干扰。该方法从添加到样品中的茶碱回收率、精密度以及干血斑结果与血浆样品结果的相关性等方面进行了研究。还研究了高压灭菌干血斑洗脱液中其他一些药物的回收率。
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