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采用全自动酶免疫分析技术(EMIT)监测血清茶碱浓度。

Monitoring of serum theophylline concentrations by a fully mechanized enzyme immunoassay (EMIT).

作者信息

Oellerich M, Sybrecht G W, Haeckel R

出版信息

J Clin Chem Clin Biochem. 1979 May;17(5):299-302. doi: 10.1515/cclm.1979.17.5.299.

Abstract

An evaluation of an enzyme immunoassay for the determination of theophylline in serum (EMIT, Syva Corp.) is presented. The EMIT assay was fully mechanized by the use of an Eppendorf analyzer 5010. The precision of this enzyme immunoassay was satisfactory (coefficients of variation from day to day ranged from 5--6%). The EMIT assay proved to be specific, rapid and easy to perform. A comparison of the results obtained by EMIT and ultraviolet spectrophotometry in a series of 100 patients showed a good correlation between both methods.

摘要

本文介绍了一种用于测定血清中茶碱的酶免疫分析方法(EMIT,Syva公司)的评估。通过使用Eppendorf 5010分析仪,EMIT分析实现了完全自动化。这种酶免疫分析方法的精密度令人满意(日间变异系数在5%-6%之间)。EMIT分析方法具有特异性、快速且易于操作。对100例患者采用EMIT法和紫外分光光度法所得结果进行比较,结果显示两种方法具有良好的相关性。

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