Aarhus University Centre for Water Technology, Section for Microbiology, Department of Bioscience , Aarhus University , Ny Munkegade , DK-8000 Aarhus C , Denmark.
Marine Biological Section, Department of Biology , University of Copenhagen , Strandpromenaden 5 , DK-3000 Helsingør , Denmark.
Anal Chem. 2019 Mar 5;91(5):3233-3238. doi: 10.1021/acs.analchem.8b05869. Epub 2019 Feb 20.
Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.
基于荧光寿命的成像是使用化学传感器技术生成化学图像最可靠的方法。然而，只有少数商业系统能够在相关的纳秒到微秒范围内实现寿命成像。在本技术说明中，我们比较了基于时域（TD）的相机系统和基于频域（FD）的相机系统在环境样品中的 O 和 pH 成像以及在可见和近红外光谱部分发射的不同指示剂染料系统的测量特性和适用性方面的性能。我们的结论是，新引入的 FD 成像系统提供了可比的（如果不是更好的）结果，优于其前身，现在能够基于 FD 荧光寿命测量实现稳健且简单的化学成像。
