Max Planck Institute for Marine Microbiology, 28359 Bremen, Germany.
Science for Life Laboratory, Department of Organismal Biology, Uppsala University, Norbyvägen 18A, SE-752 36 Uppsala, Sweden.
Cell Rep Methods. 2022 May 23;2(5):100216. doi: 10.1016/j.crmeth.2022.100216.
From individual cells to whole organisms, O transport unfolds across micrometer- to millimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O dynamics via currently available methods difficult or unreliable. Here, we present "sensPIV," a method to simultaneously measure O concentrations and flow fields. By tracking O-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, we measured O transport within (1) microfluidic devices, (2) sinking model aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliary movement to link zones of photosynthetic O production to zones of O consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.
从单个细胞到整个生物体，氧气传输跨越了从微米到毫米的长度尺度，并能在毫秒内响应流体流动和生物体行为而变化。这些过程的时空复杂性使得通过当前可用的方法准确评估氧气动力学变得困难或不可靠。在这里，我们提出了“sensPIV”方法，该方法可以同时测量氧气浓度和流场。通过使用允许即时参考的成像技术在流动中跟踪对氧气敏感的微粒子，我们测量了（1）微流控设备、（2）下沉模型聚集体和（3）复杂的形成菌落的珊瑚内的氧气传输。通过使用 sensPIV，我们发现珊瑚利用纤毛运动将光合作用产生氧气的区域与氧气消耗的区域连接起来。sensPIV 有可能扩展到研究许多生命科学和工程应用中的流动-生物体相互作用。
