a Infectious Disease Research Unit, Department of Translational Medicine , Lund University , Malmö , Sweden.
b Department of Clinical Microbiology, Division of Laboratory Medicine , Lund University , Lund , Sweden.
Infect Dis (Lond). 2019 Apr;51(4):241-248. doi: 10.1080/23744235.2018.1546055. Epub 2019 Feb 14.
Along with the current development of molecular diagnostic methods of respiratory viruses, the bedside patient sampling techniques need to be evaluated. We here asked the question whether the addition of an oropharynx swab to the traditional nasopharynx swab might improve the diagnostic yield of multiplex PCR analysis. Ct values from the two sampling sites were compared as well as patient tolerability.
In an emergency department in Malmö, Sweden, 98 adult patients with respiratory disease were sampled both from the nasopharynx and oropharynx for virus diagnostics by PCR.
Influenza (AH1, AH3, B), human metapneumovirus (hMPV) or respiratory syncytial virus (RSV) were detected by PCR in 58 subjects. The diagnostic yield was improved by combining nasopharyngeal and oropharyngeal sampling - a virus was detected in another 6 patients compared to traditional nasopharyngeal sampling (p = .031, McNemar's test). In 38/55 subjects viral load was higher in the nasopharynx than in the oropharynx. Self-reported discomfort was significantly lower from oropharyngeal sampling than from nasopharyngeal sampling.
Adding an oropharynx sample to a nasopharynx sample increased the diagnostic yield of respiratory viruses. Oropharyngeal sampling was well tolerated.
随着呼吸道病毒分子诊断方法的当前发展，需要评估床边患者采样技术。我们在这里询问了一个问题，即在传统的鼻咽拭子中添加口咽拭子是否可以提高多重 PCR 分析的诊断率。比较了两个采样部位的 Ct 值以及患者的耐受性。
在瑞典马尔默的一个急诊科，98 名患有呼吸道疾病的成年患者通过 PCR 同时从鼻咽和口咽采集样本进行病毒诊断。
PCR 检测到 58 例流感（AH1、AH3、B）、人偏肺病毒（hMPV）或呼吸道合胞病毒（RSV）。与传统的鼻咽取样相比，联合鼻咽和口咽取样可提高诊断率 - 与传统的鼻咽取样相比，又检测到另外 6 例病毒（p = .031，McNemar 检验）。在 38/55 例患者中，病毒载量在鼻咽中高于口咽。口咽取样的自我报告不适感明显低于鼻咽取样。
在鼻咽样本中添加口咽样本可提高呼吸道病毒的诊断率。口咽取样的耐受性良好。
