Naujoks H, Siracky J, Drahovsky D, Pfeifer G P
Department of Clinical Cytology, Klinikum University, Frankfurt, Germany.
Diagn Cytopathol. 1988;4(3):230-3. doi: 10.1002/dc.2840040310.
Enzymatic methylation of mammalian DNA is closely related to the replication process; also, the synthesis of DNA methylase, an enzyme that is responsible for this process, is cell cycle related. A monoclonal antibody against DNA methylase recognizes proliferatively active cells in the heterogeneous population. We used this antibody to identify the proliferative state of different cell types in normal vaginal smears and in smears of patients with precancerous lesions and cervical cancer. In all preparations, the normal epithelial cells remained unstained. The majority of cancer cells gave a positive immunocytochemical signal indicating the presence of DNA methylase antigen and the proliferative state. Staining was also observed in dyskaryotic cells, particularly in nuclei of parabasal-type cells.
哺乳动物DNA的酶促甲基化与复制过程密切相关；此外，负责此过程的酶——DNA甲基化酶的合成也与细胞周期相关。一种针对DNA甲基化酶的单克隆抗体可识别异质群体中增殖活跃的细胞。我们使用这种抗体来鉴定正常阴道涂片以及癌前病变和宫颈癌患者涂片内不同细胞类型的增殖状态。在所有标本中，正常上皮细胞均未染色。大多数癌细胞给出了阳性免疫细胞化学信号，表明存在DNA甲基化酶抗原及增殖状态。在核异质细胞中也观察到了染色，尤其是在基底旁型细胞的细胞核中。
